The enteric pathogen Cryptosporidium parvum exports proteins into the cytosol of the infected host cell

• DOI: 10.7554/elife.70451
• Publication Date: 2021-12-06T12:15:25Z
• Authors: Jennifer E Dumaine; Adam Sateriale; Alexis R Gibson; Amita G Reddy; Jodi A Gullicksrud; Emma N Hunter; Joseph T Clark; Boris Striepen
• Keywords: Cryptosporidium parvum; Enterocyte; Intracellular parasite

The parasite Cryptosporidium is responsible for diarrheal disease in young children causing death, malnutrition, and growth delay. invades enterocytes where it develops a unique intracellular niche. Infected cells exhibit profound changes morphology, physiology, transcriptional activity. How the effects these poorly understood. We explored localization of highly polymorphic proteins found members parvum MEDLE protein family to be translocated into cytosol infected cells. All life stages engage this export, which occurs after completion invasion. Mutational studies defined an N-terminal host-targeting motif demonstrated proteolytic processing at specific leucine residue. Direct expression MEDLE2 mammalian triggered ER stress response, was also observed during infection. Taken together, our reveal presence secretion system capable delivering enterocyte.