Legionella pneumophila modulates host energy metabolism by ADP-ribosylation of ADP/ATP translocases

• DOI: 10.7554/elife.73611
• Publication Date: 2022-01-27T12:15:27Z
• Authors: Jiaqi Fu; Mowei Zhou; Marina A Gritsenko; Ernesto S Nakayasu; Lei Song; Zhao-Qing Luo
• Keywords: ADP ribosylation factor; Adenosine triphosphate

The intracellular pathogen Legionella pneumophila delivers more than 330 effectors into host cells by its Dot/Icm secretion system. Those direct the biogenesis of -containing vacuole (LCV) that permits survival and replication. It has long been documented LCV is associated with mitochondria a number have shown to target this organelle. Yet, biochemical function cell most these remain unknown. Here, we found substrate Ceg3 (Lpg0080) mono-ADP-ribosyltransferase localizes in where it attacks ADP/ATP translocases ADP-ribosylation, blunts their exchange activity. modification occurs on second arginine residue -RRRMMM- element, which conserved among all known carriers from different organisms. Our results reveal modulation energy metabolism as virulence mechanism for L. .