Manganese-enhanced magnetic resonance imaging reveals light-induced brain asymmetry in embryo

• DOI: 10.7554/elife.86116
• Publication Date: 2023-09-05T13:20:11Z
• Authors: Elena Lorenzi; Stefano Tambalo; Giorgio Vallortigara; Angelo Bifone
• Keywords: In ovo

The idea that sensory stimulation to the embryo (in utero or in ovo) may be crucial for brain development is widespread. Unfortunately, up now evidence was only indirect because mapping of embryonic activity vivo challenging. Here, we applied first time manganese enhanced magnetic resonance imaging (MEMRI), a functional method, eggs domestic chicks. We revealed light-induced asymmetry by comparing were stimulated light maintained darkness. Our protocol paves way investigation effects variety stimulations on embryo.