Single-cell RNA sequencing unravels the transcriptional network underlying zebrafish retina regeneration

• DOI: 10.7554/elife.86507
• Publication Date: 2023-04-12T07:51:00Z
• Authors: Laura Celotto; Fabian Rost; Anja Machate; Juliane Bläsche; Andreas Dahl; Anke Weber; Stefan Hans; Michael Brand
• Keywords: Muller glia; Cell fate determination; Progenitor; Retinal regeneration

In the lesioned zebrafish retina, Müller glia produce multipotent retinal progenitors that generate all neurons, replacing lost cell types. To study molecular mechanisms linking reactivity to progenitor production and neuronal differentiation, we used single-cell RNA sequencing of glia, regenerated progeny from uninjured light-lesioned retinae. We discover an injury-induced differentiation trajectory leads into a population with hybrid identity expressing marker genes progenitors. A glial self-renewal neurogenic depart population. further observe progressively differentiate ganglion cells first bipolar last, similar events observed during development. Our work provides comprehensive description transcriptional changes fate decisions in regenerating which are key tailor replacement therapies for dystrophies humans.