A simple mechanism for integration of quorum sensing and cAMP signalling in V. cholerae

• DOI: 10.7554/elife.86699.2
• Publication Date: 2023-06-08T14:13:23Z
• Authors: Lucas M. Walker; James R.J. Haycocks; Julia C. van Kessel; Triana N. Dalia; Ankur B. Dalia; David C. Grainger
• Keywords: Signalling

Abstract Many bacteria use quorum sensing to control changes in lifestyle. The process is regulated by microbially derived “autoinducer” signalling molecules, that accumulate the local environment. Individual cells sense autoinducer abundance, infer population density, and alter their behaviour accordingly. In Vibrio cholerae, signals are transduced phosphorelay transcription factor LuxO. Unphosphorylated LuxO permits expression of HapR, which alters global gene patterns. this work, we have mapped genome-wide distribution HapR V. cholerae. Whilst has a small regulon, targets 32 loci. coincide with sites for cAMP receptor protein (CRP) regulates transcriptional response carbon starvation. This overlap, also evident other species, results from similarities DNA sequence bound each factor. At shared sites, CRP simultaneously contact double helix binding stabilised direct interaction two factors. Importantly, involves surface usually contacts RNA polymerase stimulate transcription. As result, can block activation CRP. Thus, interacting at integrate information expression. likely allows cholerae regulate subsets genes during transition between aquatic environments human host.