The Mycobacterium ulcerans toxin mycolactone causes destructive Sec61-dependent loss of the endothelial glycocalyx and vessel basement membrane to drive skin necrosis

• DOI: 10.7554/elife.86931.3
• Publication Date: 2025-02-06T15:50:55Z
• Authors: Louise Tzung-Harn...; Belinda S Hall; Jane Newcombe; Tom A Mendum; Sonia Santana Varela; Yagnesh Umrania; Michael J Deery; Wei Q Shi; Josué Diaz-Delgado; Francisco J Salguero; Rachel E Simmonds
• Keywords: endothelium; QH301-705.5; infectious disease; Science; Bacterial Toxins; mycolactone; mycobacterium ulcerans; Glycocalyx; Basement Membrane; Mice; Necrosis; Humans; Animals; human; Biology (General); Buruli Ulcer; mouse; Cells, Cultured; Skin; Microbiology and Infectious Disease; Mycobacterium ulcerans; microbiology; Q; R; Endothelial Cells; Sec61; basement membrane; 3. Good health; Disease Models, Animal; Medicine; Macrolides; microvasculature

The drivers of tissue necrosis in Mycobacterium ulcerans infection (Buruli ulcer disease) have historically been ascribed solely to the directly cytotoxic action of the diffusible exotoxin, mycolactone. However, its role in the clinically evident vascular component of disease aetiology remains poorly explained. We have now dissected mycolactone’s effects on human primary vascular endothelial cells in vitro. We show that mycolactone-induced changes in endothelial morphology, adhesion, migration, and permeability are dependent on its action at the Sec61 translocon. Unbiased quantitative proteomics identified a profound effect on proteoglycans, driven by rapid loss of type II transmembrane proteins of the Golgi, including enzymes required for glycosaminoglycan (GAG) synthesis, combined with a reduction in the core proteins themselves. Loss of the glycocalyx is likely to be of particular mechanistic importance, since knockdown of galactosyltransferase II (beta-1,3-galactotransferase 6; B3GALT6), the GAG linker-building enzyme, phenocopied the permeability and phenotypic changes induced by mycolactone. Additionally, mycolactone depleted many secreted basement membrane components and microvascular basement membranes were disrupted in vivo during M. ulcerans infection in the mouse model. Remarkably, exogenous addition of laminin-511 reduced endothelial cell rounding, restored cell attachment and reversed the defective migration caused by mycolactone. Hence supplementing mycolactone-depleted extracellular matrix may be a future therapeutic avenue, to improve wound healing rates.