Switching of RNA splicing regulators in immature neuroblasts during adult neurogenesis

• DOI: 10.7554/elife.87083.3
• Publication Date: 2024-11-22T16:10:42Z
• Authors: Corentin Bernou; Marc-André Mouthon; Mathieu Daynac; Thierry Kortulewski; Benjamin Demaille; Vilma Barroca; Sebastien Couilla...; Nathalie Dechamps; Véronique Ménard; Léa Bellenger; Christophe Antoni...; Alexandra Déborah...; François Dominiqu...
• Keywords: Neuroblast

The lateral wall of the mouse subventricular zone harbors neural stem cells (NSC, B cells) which generate proliferating transient-amplifying progenitors (TAP, C that ultimately give rise to neuroblasts (NB, A cells). Molecular profiling at single-cell level struggles distinguish these different cell types. Here, we combined transcriptome analyses FACS-sorted and RNAseq demonstrate existence an abundant, clonogenic multipotent population immature (iNB transition between TAP migrating NB (mNB). iNB are reversibly engaged in neuronal differentiation. Indeed, they keep molecular features both undifferentiated progenitors, plasticity unexpected regenerative properties. Strikingly, undergo important progressive switches, including changes expression splicing regulators leading their differentiation mNB subdividing them into two subtypes, iNB1 iNB2. Due plastic properties, could represent a new target for therapy brain damage.