High-precision mapping of nuclear pore-chromatin interactions reveals new principles of genome organization at the nuclear envelope

• DOI: 10.7554/elife.87462
• Publication Date: 2023-06-23T21:40:07Z
• Authors: Swati Tyagi; Juliana S. Capitanio; Jiawei Xu; Fei Chen; Rahul Sharma; Jialiang Huang; Martin W. Hetzer
• Keywords: Nuclear pore; Nucleoporin; Genomic Organization; Nuclear lamina

The role of nuclear pore complexes (NPCs) in genome organization remains poorly characterized due to technical limitations probing genome-wide protein-DNA interactions specific the periphery. Here, we developed a new sensitive method, NPC-DamID, which combines vitro reconstitution import and DamID technology. fixation-free method identifies chromatin at NPCs intact nuclei from cells tissues. We found that are preferentially associated with common hierarchically arranged super-enhancers (SEs) across multiple cell types. also uncovered phase-separated condensates compartmentalize concentrate transcriptional coactivators structural proteins SE-regulated genes. Our results support as anchoring sites for SE regulatory hubs cell-type-specific control.