Identification of Novel Syncytiotrophoblast Membrane Extracellular Vesicles Derived Protein Biomarkers in Early-onset Preeclampsia: A Cross-Sectional Study

• DOI: 10.7554/elife.88841.2
• Publication Date: 2024-04-08T11:25:09Z
• Authors: Toluwalase Awoyemi; Shuhan Jiang; Bríet Bjarkadóttir; Maryam Rahbar; Prasanna Logenthiran; Gavin Collett; Wei Zhang; Adam Cribbs; Ana Sofia Cerdeira; Manu Vatish
• Keywords: Syncytiotrophoblasts; Extracellular Vesicles; Identification; Cross-sectional study

Preeclampsia (PE), a multi-systemic hypertensive pregnancy disease that affects 2-8% of pregnancies worldwide, is leading cause adverse maternal and fetal outcomes. Current clinical PE tests have low positive predictive value for prediction diagnosis. The placenta notably releases extracellular vesicles from the syncytiotrophoblast (STB-EV) into circulation.To identify difference in STB-EV proteome between normal (NP), which could lead to identifying potential biomarkers mechanistic insights.Using ex-vivo dual lobe perfusion, we performed mass spectrometry on placental tissue, medium/large small STB-EVs isolated (n = 6) NP placentae. Bioinformatically, was used differentially carried proteins. Western blot validate identified biomarkers. We finished our investigation with an in-silico pathways.We NP. Filamin B, collagen 17A1, pappalysin-A2, scavenger Receptor Class B Type 1) were discovered verified different abundances compared In silico revealed novel processes (such as abnormal protein metabolism) may contribute pathological manifestations PE.We potentially pathways proteins be important pathophysiology are worth investigating because they future studies mechanisms biomarkers.This research funded by Medical Research Council (MRC Programme Grant (MR/J0033601) & Life Sciences translational fund (BRR00142 HE01.01)