Trabid patient mutations impede the axonal trafficking of adenomatous polyposis coli to disrupt neurite growth

• DOI: 10.7554/elife.90796
• Publication Date: 2023-10-11T15:48:11Z
• Authors: Daniel Frank; Maria Bergamasco; Michael J Mlodzia...; Andrew Kueh; Ellen Tsui; Cathrine Hall; Georgios Kastrappis; Anne Kathrin Voss; Catriona McLean; Maree Faux; Kelly L Rogers; Bang Tran; Elizabeth Vincan; David Komander; Grant Dewson; Hoanh Tran
• Keywords: 570; Trabid; neurodevelopment; QH301-705.5; adenomatous polyposis coli; Science; Q; Adenomatous Polyposis Coli Protein; R; 610; Cell Biology; STRIPAK; Axons; ZRANB1; Mice; Adenomatous Polyposis Coli; Mutation; Neurites; Medicine; Animals; Humans; Biology (General); Child; axonal protein trafficking

ZRANB1 (human Trabid) missense mutations have been identified in children diagnosed with a range of congenital disorders including reduced brain size, but how Trabid regulates neurodevelopment is not understood. We have characterized these patient mutations in cells and mice to identify a key role for Trabid in the regulation of neurite growth. One of the patient mutations flanked the catalytic cysteine of Trabid and its deubiquitylating (DUB) activity was abrogated. The second variant retained DUB activity, but failed to bind STRIPAK, a large multiprotein assembly implicated in cytoskeleton organization and neural development. Zranb1 knock-in mice harboring either of these patient mutations exhibited reduced neuronal and glial cell densities in the brain and a motor deficit consistent with fewer dopaminergic neurons and projections. Mechanistically, both DUB-impaired and STRIPAK-binding-deficient Trabid variants impeded the trafficking of adenomatous polyposis coli (APC) to microtubule plus-ends. Consequently, the formation of neuronal growth cones and the trajectory of neurite outgrowth from mutant midbrain progenitors were severely compromised. We propose that STRIPAK recruits Trabid to deubiquitylate APC, and that in cells with mutant Trabid, APC becomes hyperubiquitylated and mislocalized causing impaired organization of the cytoskeleton that underlie the neuronal and developmental phenotypes.