TRIP13 localizes to synapsed chromosomes and functions as a dosage-sensitive regulator of meiosis

• DOI: 10.7554/elife.92195
• Publication Date: 2023-11-22T11:22:40Z
• Authors: Jessica Y Chotiner; N Adrian Leu; Fang Yang; Isabella G Cossu; Yongjuan Guan; Huijuan Lin; P Jeremy Wang
• Keywords: fertility; Male; Mice, Knockout; TRIP13; QH301-705.5; Synaptonemal Complex; Science; Q; R; Cell Cycle Proteins; Cell Biology; Telomere; spermatogenesis; Article; Mice; Meiosis; Chromosome Pairing; Spermatocytes; meiosis; Medicine; Animals; ATPases Associated with Diverse Cellular Activities; Female; Biology (General)

Meiotic progression requires coordinated assembly and disassembly of protein complexes involved in chromosome synapsis meiotic recombination. Mouse TRIP13 its ortholog Pch2 are instrumental remodeling HORMA domain proteins. HORMAD proteins associated with unsynapsed axes but depleted from the synaptonemal complex (SC) synapsed homologs. Here we report that localizes to SC early pachytene spermatocytes telomeres throughout prophase I. Loss leads arrest thus sterility both sexes. Trip13 -null meiocytes exhibit abnormal persistence HORMAD1 HOMRAD2 on asynapsis preferentially affects XY centromeric ends. These major phenotypes consistent reported hypomorph alleles. heterozygous mice defects less severe than mice, showing is a dosage-sensitive regulator meiosis. Localization independent axial element such as REC8 SYCP2/SYCP3. Terminal FLAG-tagged functional recapitulate localization native telomeres. Therefore, evolutionarily conserved TRIP13/Pch2 chromosomes provides an explanation for dissociation upon diverse organisms.