Bacteria are a major determinant of Orsay virus transmission and infection in Caenorhabditis elegans

• DOI: 10.7554/elife.92534.3
• Publication Date: 2024-07-11T16:51:53Z
• Authors: Brian G Vassallo; Noemie Scheidel; Sylvia E J Fischer; Dennis H Kim
• Keywords: Pathogenic bacteria

The microbiota is a key determinant of the physiology and immunity animal hosts. factors governing transmissibility viruses between susceptible hosts are incompletely understood. Bacteria serve as food for Caenorhabditis elegans represent an integral part natural environment C. . We determined effects bacteria isolated with from its on transmission Orsay virus in using quantitative host susceptibility assays. observed that Ochrobactrum species promoted transmission, whereas Pseudomonas lurida MYb11 attenuated relative to standard laboratory bacterial Escherichia coli OP50. found pathogenic aeruginosa strains PA01 PA14 further transmission. amount required infect 50% population P. compared vermis MYb71 was dramatically increased, over three orders magnitude. Host even presence PA14. Genetic analysis determinants attenuation infection revealed role regulators quorum sensing. Our data suggest distinct constituents potential pathogens can have widely divergent such associated effectively determine versus resistance viral infection. study provides evidence critical tripartite host-virus-bacteria interactions determining among