Noncaloric monosaccharides induce excessive sprouting angiogenesis in zebrafish via foxo1a-marcksl1a signal

• DOI: 10.7554/elife.95427.1
• Publication Date: 2024-04-03T15:25:15Z
• Authors: Xiaoning wang; Jinxiang Zhao; Jiehuan Xu; Bowen Li; Xia Liu; Gangcai Xie; Xuchu Duan; Dong Liu
• Keywords: Sprouting; Monosaccharide

Artificially sweetened beverages containing noncaloric monosaccharides were suggested as healthier alternatives to sugar-sweetened beverages. Nevertheless, the potential detrimental effects of these on blood vessel function remain inadequately understood. Presently, we have established a zebrafish model that exhibits significant excessive angiogenesis induced by high glucose, resembling hyperangiogenic characteristics observed in proliferative diabetic retinopathy (PDR). Utilizing this model, glucose and could induce formation vessels, especially intersegmental vessels (ISVs). The excessively branched be formed ectopic activation quiescent endothelial cells (ECs) into tip cells. Single-cell transcriptomic sequencing analysis embryos exposed revealed an augmented ratio capillary ECs, proliferating series upregulated proangiogenic genes. Further experiments validated foxo1a mediated down-regulating expression marcksl1a . This study has provided new evidence showing negative vascular system underlying mechanisms.