A split-GAL4 driver line resource for Drosophila neuron types
Neurons
Central Nervous System
D. melanogaster
QH301-705.5
Science
Q
R
central nervous system
confocal microscopy
neuroscience
Animals, Genetically Modified
Drosophila melanogaster
driver line
Larva
Animals
Drosophila Proteins
GAL4
Medicine
split-GAL4
Biology (General)
targeting
Transcription Factors
Neuroscience
DOI:
10.7554/elife.98405.2
Publication Date:
2025-01-06T14:25:15Z
AUTHORS (84)
ABSTRACT
Abstract
Techniques that enable precise manipulations of subsets of neurons in the fly central nervous system have greatly facilitated our understanding of the neural basis of behavior. Split-GAL4 driver lines allow specific targeting of cell types in Drosophila melanogaster and other species. We describe here a collection of 3060 lines targeting a range of cell types in the adult Drosophila central nervous system and 1373 lines characterized in third-instar larvae. These tools enable functional, transcriptomic, and proteomic studies based on precise anatomical targeting. NeuronBridge and other search tools relate light microscopy images of these split-GAL4 lines to connectomes reconstructed from electron microscopy images. The collections are the result of screening over 77,000 split hemidriver combinations. Previously published and new lines are included, all validated for driver expression and curated for optimal cell type specificity across diverse cell types. In addition to images and fly stocks for these well-characterized lines, we make available 300,000 new 3D images of other split-GAL4 lines.
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (91)
CITATIONS (0)
EXTERNAL LINKS
PlumX Metrics
RECOMMENDATIONS
FAIR ASSESSMENT
Coming soon ....
JUPYTER LAB
Coming soon ....