A split-GAL4 driver line resource for Drosophila neuron types

• DOI: 10.7554/elife.98405.2
• Publication Date: 2025-01-06T14:25:15Z
• Authors: Geoffrey W Meissner; Allison Vannan; Jennifer Jeter; Kari Close; Gina M DePasquale; Zachary Dorman; Kaitlyn Forster; Jaye Anne Beringer; Theresa V Gibney; Joanna H Hausenfluck; Yisheng He; Kristin Henderson; Lauren Johnson; Rebecca M Johnston; Gudrun Ihrke; Nirmala Iyer; Rachel Lazarus; Kelley Lee; Hsing-Hsi Li; Hua-Peng Liaw; Brian Melton; Scott Miller; Reeham Motaher; Alexandra Novak; Omotara Ogundeyi; Alyson Petruncio; Jacquelyn Price; Sophia Protopapas; Susana Tae; Jennifer Taylor; Rebecca Vorimo; Brianna Yarbrough; Kevin Xiankun Zeng; Christopher T Zug...; Heather Dionne; Claire Angstadt; Kelly Ashley; Amanda Cavallaro; Tam Dang; Guillermo A Gonzalez; Karen L Hibbard; Cuizhen Huang; Jui-Chun Kao; Todd Laverty; Monti Mercer; Brenda Perez; Scarlett Pitts; Danielle Ruiz; Viruthika Vallanadu; Grace Zhiyu Zheng; Cristian Goina; Hideo Otsuna; Konrad Rokicki; Robert R Svirskas; Han SJ Cheong; Michael-John Dolan; Erica Ehrhardt; Kai Feng; Basel El Galfi; Jens Goldammer; Stephen J Huston; Nan Hu; Masayoshi Ito; Claire McKellar; Ryo Minegishi; Shigehiro Namiki; Aljoscha Nern; Catherine E Schre...; Gabriella R Sterne; Lalanti Venkatasu...; Kaiyu Wang; Tanya Wolff; Ming Wu; Reed George; Oz Malkesman; Yoshinori Aso; Gwyneth M Card; Barry J Dickson; Wyatt Korff; Kei Ito; James W Truman; Marta Zlatic; Gerald M Rubin
• Keywords: Neurons; Central Nervous System; D. melanogaster; QH301-705.5; Science; Q; R; central nervous system; confocal microscopy; neuroscience; Animals, Genetically Modified; Drosophila melanogaster; driver line; Larva; Animals; Drosophila Proteins; GAL4; Medicine; split-GAL4; Biology (General); targeting; Transcription Factors; Neuroscience

Abstract Techniques that enable precise manipulations of subsets of neurons in the fly central nervous system have greatly facilitated our understanding of the neural basis of behavior. Split-GAL4 driver lines allow specific targeting of cell types in Drosophila melanogaster and other species. We describe here a collection of 3060 lines targeting a range of cell types in the adult Drosophila central nervous system and 1373 lines characterized in third-instar larvae. These tools enable functional, transcriptomic, and proteomic studies based on precise anatomical targeting. NeuronBridge and other search tools relate light microscopy images of these split-GAL4 lines to connectomes reconstructed from electron microscopy images. The collections are the result of screening over 77,000 split hemidriver combinations. Previously published and new lines are included, all validated for driver expression and curated for optimal cell type specificity across diverse cell types. In addition to images and fly stocks for these well-characterized lines, we make available 300,000 new 3D images of other split-GAL4 lines.