A split-GAL4 driver line resource for Drosophila neuron types

• DOI: 10.7554/elife.98405.3
• Publication Date: 2025-01-24T16:55:42Z
• Authors: Geoffrey W Meissner; Allison Vannan; Jennifer Jeter; Kari Close; Gina M DePasquale; Zachary Dorman; Kaitlyn Forster; Jaye Anne Beringer; Theresa Gibney; Joanna H Hausenfluck; Yisheng He; Kristin Henderson; Lauren Johnson; Rebecca M Johnston; Gudrun Ihrke; Nirmala A Iyer; Rachel Lazarus; Kelley Lee; Hsing-Hsi Li; Hua-Peng Liaw; Brian Melton; Scott Miller; Reeham Motaher; Alexandra Novak; Omotara Ogundeyi; Alyson Petruncio; Jacquelyn Price; Sophia Protopapas; Susana Tae; Jennifer Taylor; Rebecca Vorimo; Brianna Yarbrough; Kevin Xiankun Zeng; Christopher T Zug...; Heather Dionne; Claire Angstadt; Kelly Ashley; Amanda Cavallaro; Tam Dang; Guillermo A Gonzalez; Karen L Hibbard; Cuizhen Huang; Jui-Chun Kao; Todd Laverty; Monti Mercer; Brenda Perez; Scarlett Rose Pitts; Danielle Ruiz; Viruthika Vallanadu; Grace Zhiyu Zheng; Cristian Goina; Hideo Otsuna; Konrad Rokicki; Robert R Svirskas; Han SJ Cheong; Michael-John Dolan; Erica Ehrhardt; Kai Feng; Basel EI Galfi; Jens Goldammer; Stephen J Huston; Nan Hu; Masayoshi Ito; Claire McKellar; Ryo Minegishi; Shigehiro Namiki; Aljoscha Nern; Catherine E Schre...; Gabriella R Sterne; Lalanti Venkatasu...; Kaiyu Wang; Tanya Wolff; Ming Wu; Reed George; Oz Malkesman; Yoshinori Aso; Gwyneth M Card; Barry J Dickson; Wyatt Korff; Kei Ito; James W Truman; Marta Zlatic; Gerald M Rubin
• Keywords: Line (geometry)

Techniques that enable precise manipulations of subsets neurons in the fly central nervous system (CNS) have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult CNS 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. Previously published new included, all validated for expression curated optimal cell-type specificity across diverse types. In addition stocks well-characterized lines, we make available 300,000 3D lines.