Analysis of microRNA expression profiles in exosomes derived from acute myeloid leukemia by p62 knockdown and effect on angiogenesis

• DOI: 10.7717/peerj.13498
• Publication Date: 2022-07-22T08:21:07Z
• Authors: Chuan Li; Xinyi Long; Peiqi Liang; Zhuogang Liu; Chen Wang; Rong Hu
• Keywords: KEGG; Exosome

Objectives In this study, we aimed to investigate the effect of p62 on angiogenesis and microRNA (miRNA) expression profiles in acute myeloid leukemia (AML) exosomes. Methods An Exiqon v19.0 MicroArray was used profile miRNAs exosomes derived from parental p62-knockdown U937 cells. The Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) databases were predict biological functions potential mechanisms differentially expressed AML Endothelial cell tube formation assays using human umbilical vein endothelial cells (HUVECs) performed angiogenesis. Results We demonstrated that 2,080 our cultured samples, which 215 208 upregulated downregulated, respectively, (fold change ≥ 2, P < 0.05). GO analysis indicated most enriched intercellular pathways. Biological process revealed 1460 processes associated with downregulated transcripts, including 19 pathways related vesicles, 1,515 upregulated, 8 vesicles. Molecular function protein binding, transcription regulator activity, DNA-binding factor activity ( Pathway 84 corresponded 55 transcripts also found promoted HUVECs. Conclusions Our data suggest exosomal may play important roles pathogenesis AML, be treated by knockdown inhibit