Identification and validation of immune related core transcription factors GTF2I in NAFLD

• DOI: 10.7717/peerj.13735
• Publication Date: 2022-07-21T08:25:00Z
• Authors: Minbo Zhang; Yu Zhang; Xiaoxiao Jiao; Linying Lai; Yiting Qian; Bo Sun; Wenzhuo Yang
• Keywords: 0303 health sciences; QH301-705.5; Chemokine receptor family; R; Biochemistry; 3. Good health; Immune; Transcription Factors, TFII; 03 medical and health sciences; Non-alcoholic Fatty Liver Disease; Transcription Factors, TFIII; NAFLD; Medicine; Humans; Receptors, Chemokine; GTF2I; Biology (General); TF; Biomarkers; Transcription Factors

Background Nonalcoholic fatty liver disease (NAFLD) is the most common worldwide that endangers human health. Transcription factors (TFs) have gradually become hot spots for drug development in NAFLD their impacts on metabolism. However, specific TFs regulate immune response of not clear. This study aimed to investigate involved and provide novel targets development. Methods Microarray data were obtained from samples 26 normal volunteers 109 patients using Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) analyzed by limma package. transcription (DETFs) DEGs combined with Cistrome Cancer Immune signatures pathways hallmark identified ssGSSEA GSVA. The co-regulation network was constructed above results. Further, quantitative Real-time Polymerase Chain Reaction (qRT-PCR), Western blot (WB) Immunohistochemistry (IHC) used validate relationship between GTF2I NAFLD. CIBERSORT analysis performed identify cell types explore differential expression surface markers. Results A total 617 six DETFs ( ESR1 , CHD2 EGR1 HCFC1 SP2 ) analysis. pathway hallmarks screened through co-regulatory networks DEGs, DETFs, hallmarks. Furthermore, qRT-PCR, WB IHC indicated but significantly upregulated Finally, silico our confirmed has a wide impact profile negatively regulating chemokine receptor family (227/261, count significance). Conclusion plays role family, which affects profile. may potential target diagnosis or therapy