Antimicrobial and anti-inflammatory activity of Cystatin C on human gingival fibroblast incubated with Porphyromonas gingivalis

• DOI: 10.7717/peerj.14232
• Publication Date: 2022-10-25T07:46:20Z
• Authors: Blanca Esther Bla...; Ingeborg Becker-F...; Jaime Zamora-Chimal; José Delgado-Domí...; Adriana Ruíz-Remigio; Elba Rosa Leyva-H...; Javier Portilla-R...; Ana María Fernánd...
• Keywords: Cystatin; Proinflammatory cytokine

Background Periodontal disease is considered one of the most prevalent chronic infectious diseases, often leading to disruption tooth-supporting tissues, including alveolar bone, causing tooth mobility and loss. Porphyromonas gingivalis major etiological agent this disease, having a plethora virulence factors, including, lipopolysaccharides (LPS), hemolysins, proteinases. Antimicrobial peptides are main components innate immune response that inhibit growth P. . The aim study was analyze antimicrobial activity cystatin C assess effect on inflammatory anti-inflammatory cytokines, production reactive oxygen species, in release nitric oxide by human gingival fibroblasts incubated with presence absence C. Methods ATCC 33277 exposed for 24h co-cultured (HGFs) CRL-2014. HGFs evaluated. Pro-inflammatory (TNF α , IL-1 β ) (IL-10) cytokines were determined ELISA supernatants Additionally, nitrites species (ROS) Results Cystatin Cinhibited without affecting HGFs. Incubation led significant increase TNF- In contrast, showed decreased both whereas IL-10 enhanced. an (NO) ROS production, which reduced peptide. Conclusions inhibits decreases ROS, NO during infection Knowledge immunomodulatory properties could aid design new therapeutic approaches facilitate elimination bacterium improve treatment periodontal disease.