Identification and functional analysis of PIN family genes in Gossypium barbadense

• DOI: 10.7717/peerj.14236
• Publication Date: 2022-10-18T07:40:13Z
• Authors: Yilei Long; Quanjia Chen; Yanying Qu; Pengfei Liu; Yang Jiao; Yongsheng Cai; Xiaojuan Deng; Kai Zheng
• Keywords: Gossypium barbadense; Gossypium

Background PIN proteins are an important class of auxin polar transport that play regulatory role in plant growth and development. However, their characteristics functions have not been identified Gossypium barbadense . Methods family genes were the cotton species G. , hirsutum raimondii arboreum detailed bioinformatics analyses conducted to explore roles these using transcriptome data quantitative reverse-transcription polymerase chain reaction (qRT-PCR) technology. Functional verification was performed virus-induced gene silencing (VIGS) Results A total 138 four species; divided into seven subgroups. GbPIN members widely distributed on 20 different chromosomes, most had repeated duplication events. Transcriptome analysis showed some differential expression patterns stages fiber According ‘PimaS-7’ ‘5917’ transcript component association analysis, transcription five directly related endogenous content fibers. qRT-PCR GbPIN7 routinely expressed during development, there significant differences among materials. Transient by VIGS led significantly higher rates lower leaves stems. This study provides comprehensive Our results will form a basis for further transporter research.