FGF19 promotes cell autophagy and cisplatin chemoresistance by activating MAPK signaling in ovarian cancer

• DOI: 10.7717/peerj.14827
• Publication Date: 2023-02-02T08:43:39Z
• Authors: Wei Zhu; Meiyuan Huang; Abhimanyu Thakur; Yuanliang Yan; Xiaoying Wu
• Keywords: FGF19

Background Chemotherapy is one of the primary treatments for ovarian cancer patients. Autophagy has been linked to chemotherapy resistance in tumor cells. Recent studies have suggested that fibroblast growth factor 19 (FGF19) may be involved onset and progression malignancies. However, relationship between FGF19 autophagy still unknown. Methods Next-generation sequencing (NGS) was conducted analyze gene mutation profiles 62 cases high grade serous (HGSOC). Fluorescence situ hybridization (FISH) performed validate amplification HGSOC tissues. Quantitative PCR (qPCR) immunohistochemistry (IHC) were used difference mRNA protein expression. Meanwhile, bioinformatics techniques expression correlation with prognosis. Besides, immunofluorescence, transmission electron microscopy Cell Counting Kit 8 (CCK-8) investigate potential mechanisms. Results In this study, we found promotes cisplatin cells by inducing autophagy. NGS analysis identified a significantly amplified gene, FGF19. addition, level samples higher than normal samples. FISH results showed positive Knockdown inhibited cell through decrease LC3 Beclin 1, increase SQSTM1/p62. Furthermore, observed p38 MAPK phosphorylation down-regulated after knockdown. IFN-γ, activator, counteracted inhibition anti-proliferation effect induced knockdown Conclusion increases chemoresistance activating pathway. These could point being therapeutic target cancer.