Evaluation of waterlogging tolerance and responses of protective enzymes to waterlogging stress in pumpkin

• DOI: 10.7717/peerj.15177
• Publication Date: 2023-04-21T07:42:54Z
• Authors: Zhenwei Liu; Dandan Qiao; Zhenyu Liu; Pengwei Wang; Li Sun; Xinzheng Li
• Keywords: Waterlogging (archaeology); Malondialdehyde

Waterlogging caused by short and severe, or prolonged precipitation can be attributed to global warming. Pumpkin plants are drought-tolerant but not tolerate waterlogging stress. Under frequent rain conditions, the production of pumpkins is lower quality, sometimes rotten, harvest failure occurs in severe cases. Therefore, it great significance assess tolerance mechanism pumpkin plants. In this study, 10 novel varieties from Baimi series were used. The level was evaluated measuring coefficient biomass physiological indices using stress simulation method. criteria evaluate capacities also explored. Using principal component membership function analysis, levels ranked as follows: No. 10>; 5>; 1>; 2>; 3>; 7>; 9>; 6>; 4>; 8. Based on results, identified with strong 8 weak tolerance. responses malondialdehyde (MDA), proline, key enzymes responsible for anaerobic respiration, antioxidant studied relative expression related genes determined real-time fluorescence quantitative PCR technique. aim our study plants, thus laying a theoretical foundation breeding waterlogging-tolerant future. After flooding treatment, enzyme activities, contents proline alcohol dehydrogenases displayed an increase followed decrease. All higher than MDA gradually increased, content being 10. activities pyruvate decarboxylases (PDCs) exhibited decrease initially, increase, then again. PDC activity generally encoding superoxide dismutase, peroxidase, catalase, ascorbate peroxidase consistent their corresponding activities. During early stage stress, improved enhancing increasing