Mesenchymal stem cells alleviate sepsis-induced acute lung injury by blocking neutrophil extracellular traps formation and inhibiting ferroptosis in rats

• DOI: 10.7717/peerj.16748
• Publication Date: 2024-01-29T08:15:29Z
• Authors: TieNan Wang; Zheng Zhang; Zhizhao Deng; Weiqi Zeng; Yingxin Gao; Ziqing Hei; Dongdong Yuan
• Keywords: Neutrophil Extracellular Traps

Acute lung injury (ALI) is one of the most serious complications sepsis, characterized by high morbidity and mortality rates. Ferroptosis has recently been reported to play an essential role in sepsis-induced ALI. Excessive neutrophil extracellular traps (NETs) formation induces exacerbated inflammation crucial development In this study, we explored effects ferroptosis NETs observed therapeutic function mesenchymal stem cells (MSCs) on First, produced a cecal ligation puncture (CLP) model sepsis rats. Ferrostain-1 DNase-1 were used inhibit separately, confirm their Next, U0126 was applied suppress MEK/ERK signaling pathway, which considered be vital formation. Finally, effect MSCs CLP models. The results demonstrated that both ferrostain-1 application could improve inhibited significantly tissues, showing regulated With inhibition pathway U0126, tissues reduced, ALI improved. also had similar protective against ALI, not only inhibiting pathway-mediated formation, but alleviating tissues. We concluded protect suppressing found potential targets for treatment provided new evidence supporting clinical treatment.