Effects of time, anticoagulant and detection channel on platelet count in ethylenediaminetetraacetic acid (EDTA)-dependent pseudothrombocytopenia

• DOI: 10.7717/peerj.19103
• Publication Date: 2025-03-04T08:48:37Z
• Authors: Yu Zhang; Bei Chen; Jiaoying Feng; Wen Yu; Wei Liang; Meifen Li; Guanghua Zhai; Meina Yan
• Keywords: Ethylenediaminetetraacetic acid

EDTA-dependent pseudothrombocytopenia (EDTA-PTCP) is an in vitro phenomenon that may lead to expensive, time-consuming, and invasive diagnostic procedures as well unnecessary patient treatment. The purpose of this study was explore the effects time, anticoagulant detection channel on platelet (PLT) count EDTA-PTCP samples, suggest a better method for correcting spurious low PLT counts. In study, 43 identified samples were collected. Sysmex XN-9100, Mindray BC-6900 BC-5390 haematology analysers used test these following channels at different time points: by impedance (PLT-I), optical (PLT-O) fluorescent staining (PLT-F). time-dependent small agglutination occurred most corresponding citrate-treated samples. Our results further demonstrated significantly affected XN-9100 PLT-F exhibited greater dissociative effect than PLT-I PLT-O channels. Moreover, blood processed hematology analyzer showed highest EDTA-K2 tubes compared other data For simplest retest use automatic analyser within 30 min. addition, changing sodium citrate using 15 min also suitable