Comparison of buckwheat, red clover, and purple tansy as potential surrogate plants for use in semi-field pesticide risk assessments withBombus impatiens

• DOI: 10.7717/peerj.2228
• Publication Date: 2016-07-14T10:53:03Z
• Authors: Angela E. Gradish; G. Christopher Cu...; Andrew J. Frewin; Cynthia D. Scott-...
• Keywords: 0106 biological sciences; Ecology; QH301-705.5; Method development; R; 15. Life on land; 01 natural sciences; Bumble bee; Semi-field; Medicine; Foraging; Colony development; Biology (General); Pesticide risk assessment

Background.Bumble bees (Bombusspp.) are important wild and managed pollinators. There is increased interest in incorporating data on bumble bees into risk assessments for pesticides, but standardized methods for assessing hazards of pesticides in semi-field and field settings have not yet been established for bumble bees. During semi-field studies, colonies are caged with pesticide-treated flowering surrogate plants, which must be attractive to foragers to ensure colony exposure to the test compound, and must produce an ample nectar and pollen to sustain colonies during testing. However, it is not known which plant(s) are suitable for use in semi-field studies with bumble bees.Materials and Methods.We comparedB. impatiensforaging activity and colony development on small plots of flowering buckwheat (Fagopyrum esculentum, var. common), red clover (Trifolium pratense), and purple tansy (Phacelia tanacetifolia) under semi-field conditions to assess their suitability as surrogate plants for pesticide risk assessment studies with bumble bees. We also compared the growth characteristics and input requirements of each plant type.Results.All three plant types generally established and grew well. Red clover and purple tansy experienced significant weed pressure and/or insect pest damage. In contrast, pest pressure was extremely low in buckwheat. Overall,B. impatiensforaging activity was significantly greater on buckwheat plots than red clover or purple tansy, but plant type had no effect on number of individuals produced per colony or colony weight.Discussion.Because of the consistently high foraging activity and successful colony development observed on buckwheat plots, combined with its favourable growth characteristics and low maintenance requirements, we recommend buckwheat as a surrogate plant for use in semi-field pesticide toxicity assessments withB. impatiens.