Effect of interleukin (IL)-35 on IL-17 expression and production by human CD4+ T cells

• DOI: 10.7717/peerj.2999
• Publication Date: 2017-02-15T09:23:19Z
• Authors: Kosuke Okada; Takeki Fujimura; Takeshi Kikuchi; Makoto Aino; Yosuke Kamiya; Ario Izawa; Yuki Iwamura; Hisashi Goto; Iichiro Okabe; Eriko Miyake; Yoshiaki Hasegawa; Makio Mogi; Akio Mitani
• Keywords: Orphan receptor; T helper cell

Background Interleukin (IL)-17 produced by mainly T helper 17 (Th17) cells may play an important destructive role in chronic periodontitis (CP). Thus, anti-inflammatory cytokines, such as IL-35, might have a beneficial effect inhibiting differentiation of Th17 cells. is regulated the retinoic acid receptor-related orphan receptor (ROR) α (encoded RORA ) and ROR γ t RORC ). However, IL-35 not clear on function still incompletely understood. Therefore, we investigated effects Methods Peripheral blood mononuclear (PBMCs) were sampled from three healthy volunteers CP patients analyzed flow cytometry for cell population. differentiated cytokine cocktail (recombinant transforming growth factor- β , rIL-6, rIL-1 anti-interferon (IFN)- anti-IL-2 anti-IL-4) PBMCs cultured with or without rIL-35. IL17A (which usually refers to IL-17), mRNA expression was quantitative polymerase chain reaction, IL-17A production determined enzyme-linked immunosorbent assay. Results The proportion + CD4 slightly increased compared controls, however, there no significant differences percentage well IFN- Foxp3 between controls patients. significantly induced cocktail, induction inhibited addition rIL-35 (1 ng/mL). Discussion present study suggests that could directly suppress IL-17 via inhibition inflammatory diseases periodontitis.