Genome-wide identification of CBL family and expression analysis of CBLs in response to potassium deficiency in cotton

• DOI: 10.7717/peerj.3653
• Publication Date: 2017-08-14T03:31:51Z
• Authors: Tingting Lu; Gaofeng Zhang; Lirong Sun; Ji Wang; Fushun Hao
• Keywords: Gossypium; Gossypium barbadense

Calcineurin B-like (CBL) proteins, as calcium sensors, play pivotal roles in plant responses to diverse abiotic stresses and growth development through interaction with CBL-interacting protein kinases (CIPKs). However, knowledge about functions evolution of CBLs Gossypium plants is scarce. Here, we conducted a genome-wide survey identified 13, 13 22 CBL genes the progenitor diploid arboreum raimondii, cultivated allotetraploid hirsutum, respectively. Analysis physical properties, chromosomal locations, conserved domains phylogeny indicated rather nature among three species. Moreover, these have closer genetic evolutionary relationship from cocoa than those other plants. Most underwent under purifying selection Additionally, nearly all G. hirsutum (GhCBL) were expressed root, stem, leaf, flower fiber. Many GhCBLs preferentially while several mainly roots. Expression patterns GhCBL response potassium deficiency also studied. The expression most moderately induced roots after treatments low-potassium stress. Yeast two-hybrid experiments that GhCBL1-2, GhCBL1-3, GhCBL4-4, GhCBL8, GhCBL9 GhCBL10-3 interacted GhCIPK23, Our results provided comprehensive view valuable information for researchers further investigate functional mechanisms Gossypium.