Mycorrhization of Quercus acutissima with Chinese black truffle significantly altered the host physiology and root-associated microbiomes

• DOI: 10.7717/peerj.6421
• Publication Date: 2019-02-18T09:04:13Z
• Authors: Xiaoping Zhang; Lei Ye; Zongjing Kang; Jie Zou; Xiaoping Zhang; Xiaolin Li
• Keywords: Ectomycorrhizae; Truffle

Background Our aim was to explore how the ectomycorrhizae of an indigenous tree, Quercus acutissima, with a commercial truffle, Chinese black truffle ( Tuber indicum ), affects host plant physiology and shapes associated microbial communities in surrounding environment during early stage symbiosis. Methods To achieve this, changes root morphology microscopic characteristics, indices, rhizosphere soil properties were investigated when six-month-old synthesized. Meanwhile, next-generation sequencing technology used analyze bacterial fungal endosphere inoculated T. or not. Results The results showed that colonization by significantly improved activity superoxide dismutase roots but decreased activity. biomass, leaf chlorophyll content peroxidase did not obviously differ. Ectomycorrhization Q. acutissima affected characteristics soil, improving organic matter, total nitrogen, phosphorus available nitrogen. community composition altered colonization, as richness diversity. dominant bacteria all samples Proteobacteria Actinobacteria, fungi Eukaryota_norank, Ascomycota, Mucoromycota. Some communities, such Streptomyces, SM1A02, Rhizomicrobium more abundant ectomycorrhizosphere soil. second-most genus, Fusarium present at lower amounts . Discussion Overall, symbiotic relationship between had obvious effect on physiology, properties, which could improve our understanding indicum, may contribute cultivation truffle.