Evaluation of five regions as DNA barcodes for identification of Lepista species (Tricholomataceae, Basidiomycota) from China

• DOI: 10.7717/peerj.7307
• Publication Date: 2019-07-15T04:06:08Z
• Authors: Siyu Wang; Hongbo Guo; JiaJia Li; Wei Li; Qin Wang; Xiaodan Yu
• Keywords: Intergenic region; DNA Barcoding; Barcode

Distinguishing among species in the genus Lepista is difficult because of their similar morphologies.To identify a suitable DNA barcode for identification species, we assessed following five regions: internal transcribed spacer (ITS), intergenic (IGS), nuclear ribosomal RNA subunit, mitochondrial small subunit rDNA, and tef1. A total 134 sequences from 34 samples belong to eight were analyzed. The utility each region as was based on success rates its PCR amplification sequencing, intra- inter-specific variations.The results indicated that ITS could distinguish all tested. We therefore propose can be used Lepista. In addition, phylogenetic tree showed tested including two unrecognized formed separate well-supported clades.