Effects of ferulic acid esterase-producingLactobacillus fermentumand cellulase additives on the fermentation quality and microbial community of alfalfa silage

• DOI: 10.7717/peerj.7712
• Publication Date: 2019-10-08T08:42:29Z
• Authors: Rina Su; Kuikui Ni; Tianwei Wang; Xiaopan Yang; Jie Zhang; Yayong Liu; Weixiong Shi; Liu Yan; Chen Jie; Jin Zhong
• Keywords: Silage; Lactobacillus fermentum; Neutral Detergent Fiber

Alfalfa (Medicago sativa) is an important forage material widely used for animal feed production. Ensiling effective method preserving alfalfa, but it has shown some limitations in the production of high-quality alfalfa silage due to its low water soluble carbohydrates (WSC) content and high buffering capacity. Lactic acid bacteria (LAB) cellulase are often as additives promote ensiling process enhance fermentation quality.Experiments were conducted investigate effects ferulic esterase (FAE)-producing Lactobacillus fermentum 17SD-2 (LF) (CE) on quality microbial community silage. After 60 days ensiling, analysis bacterial diversity silages using high-performance liquid chromatography high-throughput sequencing methods.Alfalfa was ensiled with (LF, CE, LF+CE) or without days. All increased lactic decreased pH values ammonia-N contents compared control. Among all treatments, combined addition LF CE showed lowest (4.66) (NH3-N, 0.57% DM) content, highest (LA, 10.51% DM), dry matter (DM, 22.54%) crude protein (CP, 24.60% DM). Combined performed better reducing neutral detergent fiber (NDF, 29.76% (ADF, 22.86% than (33.71, 27.39% (32.07, 25.45% alone. Moreover, indicated that LF+CE treatments abundance desirable inhibited growth detrimental Enterobacter Clostridia silage.Combined FAE-producing more alone improving nutrition This likely a synergistic effect FAE produced by plant cell wall degradation, indicating these each other improve degradation fermentation. In conclusion, could be feasible way quality.