Molecular, physiological, and biochemical characterization of extracellular lipase production by Aspergillus niger using submerged fermentation

• DOI: 10.7717/peerj.9425
• Publication Date: 2020-07-07T08:15:52Z
• Authors: Amira Hassan Alab...; Norah Ayad ALanazi; Sumayh A. Aldakeel; Sayed AbdulAzeez; J. Francis Borgio
• Keywords: Aspergillus niger; Tributyrin

Extracellular production of fungal lipases especially the obtained from Aspergilli has gained immense interest in recent years due to its diverse biotechnological applications. In this study, we focused on determining fermentation parameters required for optimal lipase production.A total 256 isolates were oil seeds. From each genus, one isolate was selected evaluate using phenol red and tributyrin plate assays. Lipase activity estimated spectrophotometric pNPP hydrolysis assay. The highest producer identified 18S ribosomal RNA gene sequencing. genetic variability determined by random amplified polymorphic DNA (RAPD) analysis dendrogram constructed unweighted pair group method with arithmetic averages method. examined a submerged culture (Smf) measure effect temperature, pH, incubation time, carbon source, nitrogen inoculum volume, lipid source production.Eleven belonging genus Aspergillus analyzed where they found be producers among various genera. All tested as A. niger 18s rRNA Genetic diversity evaluated all studied RAPD primers. primers used amplify 285 loci, which five (1.75%) seven monomorphic (2.45%). Thus, high level observed isolates. test assay strains producers, their enzyme activities 709.74, 532.54, 735.64, 794.62, 787.69 U/ml. conditions follows: 40 °C, pH 7.5, 1% fructose yeast extract 2% palm oil, 2.5 × 107 spores/ml suspension, 3 days incubation.The current study provides comprehensive characterization conditions, are essential enhance