Epidemiologic Study of Diarrhea Pathogens Detected by Multiplex Real-Time PCR: a Single Center Study During 1 Year

• DOI: 10.7754/clin.lab.2022.220707
• Publication Date: 2023-02-14T21:45:56Z
• Authors: Young Kim; Min-Chul Cho
• Keywords: Multiplex; Clostridium perfringens

Acute gastroenteritis is one of the major causes morbidity and mortality worldwide, especially in children elderly. The identification various diarrhea-causing bacteria using multiplex polymerase chain reaction (PCR) rapid antigen testing has enabled a more detailed analysis pathogens. Pre-vious reports have limitation that they do not include data on multiple infections which two or infectious agents are simultaneously detected, there no clinical information. We investigated viruses detected by real-time PCR for year at single institution.This study included 766 subjects who underwent direct stool specimens purpose diagnosis from April 2019 to February 2020. test used our can detect 16 types five viruses. When pathogens were PCR, confirmed conventional PCR. Demographic, clinical, laboratory collected electronic medical records (EMR). analyzed according age season.Out total 352 samples with pathogen detection, 265 (75.3%) as 87 (24.7%) showed co-detection. highest rates co-detection Clostridium perfringens, combination co-infections was C. perfringens Staphylococcus aureus.We demonstrated different groups varying distributions. While special seasonality found monthly distribution, it should be noted number cases peaked September. presented serves epidemiologically important basic data.