Frequency of Sperm DNA Fragmentation According to Selection Method: Comparison and Relevance of a Microfluidic Device and a Swim-up Procedure

• DOI: 10.7860/jcdr/2015/10332.6811
• Publication Date: 2015-11-24T03:05:12Z
• Authors: Kanako Kishi
• Keywords: Asthenozoospermia

Multiple rounds of centrifugation or washing spermatozoa can cause sperm DNA fragmentation (SDF); however, a microfluidic approach to select does not require centrifugation. Reports have suggested that sorting using device is an effective method good quality spermatozoa, it known whether reduces damage. We investigated the frequency SDF was affected by selection during processing.Semen samples from ten men with normal, oligozoospermia and asthenozoospermia were split into two groups sorted swim-up method. Subsequently, semen parameters measured analyzed paired non-paired Student's t-tests.For (Sperm Sorter Qualis(®); Menicon, Kasugai, Japan) method, both showed decrease in SDF. However, more significant when used.Sorting resulted less than did