Antitumor Effect of Docetaxel-Loaded Lipid Microbubbles Combined With Ultrasound-Targeted Microbubble Activation on VX2 Rabbit Liver Tumors

• DOI: 10.7863/jum.2010.29.1.61
• Publication Date: 2017-01-09T22:14:22Z
• Authors: Juan Kang; Xiaoling Wu; Zhigang Wang; Haitao Ran; Chuanshan Xu; Jinfeng Wu; Zhaoxia Wang; Yong Zhang
• Keywords: MMP2

Objective. The purpose of the study was to explore antitumor effect docetaxel-loaded lipid microbubbles combined with ultrasound-targeted microbubble activation (UTMA) on VX2 rabbit liver tumors. Methods. Docetaxel-loaded were made by a mechanical vibration technique. tumor models established in 90 rabbits, which randomly divided into 6 groups, including control, docetaxal-loaded alone, docetaxal ultrasound, pure and ultrasound (DOC+MB/US). volume inhibition rate (IR) growth calculated compared. Apoptosis detected terminal deoxyuridine nick end labeling. Proliferating cell nuclear antigen matrix metalloproteinase 2 (MMP2) protein expression immunohistochemistry. Caspase 3 MMP2 messenger RNA (mRNA) situ hybridization histochemistry. metastasis survival time animals Results. IR apoptotic index DOC+MB/US group highest among all proliferating labeling lowest. Matrix mRNA lowest caspase highest. extensive lowest, longest. Conclusions. UTMA could inhibit tumors deferring proliferation promoting apoptosis, may provide novel targeted strategy for chemotherapy carcinoma.