IDENTIFICATION AND CHARACTERIZATION OF PROTEASES AND AMYLASES PRODUCING Bacillus licheniformis STRAIN EMBS026 BY 16S rRNA GENE SEQUENCING

• DOI: 10.9735/0975-5276.4.5.231-235
• Publication Date: 2014-06-23T13:08:55Z
• Authors: ANURAJ NS; SABNANI MK; MUKESH YADAV; JYOTSANA K; DEEPIKA S; RACHNA C; JYOTI SAHU
• Keywords: Bacillus licheniformis; Strain (injury); Identification

Development of natural antimicrobial strategies has always attracted researchers, as disease outbreaks are recognized im- portant constraints to living organisms because the development antibiotic resistance in microorganisms. Use probiotic bacteria been practice alternatives antimicrobials control microbial agents. Probiotics, and beneficial micro flora widely accepted used farming aquaculture its diversified applications. It improves water pond sedi- ment quality which inturn aquatic life. Bacterial pathogenicity virulence can be controlled without using excessive antibiotics help minimize risk multiple resistances. Probiotic increased productivity profits when properly they produce antagonistic compounds that inhibitory toward pathogens, compete with harmful microorganisms for nutrients energy, deleterious species adhesion sites, enhance immune response animal, improve quality, interact phytoplankton. Among large number products use today bacterial spore formers, mostly genus Bacillus. The Bacillus is extremely resistant spores provide exceptional longevity re- lease exoenzymes. current investigation identify a novel strain licheniformis by application 16S rRNA gene sequenc- ing. approach novel, proteases amylases producing detergent production. sample was isolated from near Gudiwada, Krishna district, Andhra Pradesh, India. Subsequently serially diluted aliquots were incubated suitable time period following suspected colony subjected rDNA sequencing. results showed isolate high alkaline protease bacteria, named EMBS026, after characteriza- tion sequence deposited GenBank accession JQ267798. Key words- 16s Gene sequencing, Proteases bacteria.