Antimicrobial Resistance of Escherichia coli Involved in Algerian Bovine Carriage, ESBL Detection, Integron Characterization and Genetic Lineages

• DOI: 10.9775/kvfd.2023.30670
• Publication Date: 2024-01-24T07:34:20Z
• Authors: Madjid SADI; Madjid AKKOU; Sandra MARTÍNEZ-Á...; Isabel CARVALHO; Rosa FERNÁNDEZ-FE...; Idris Nasir ABDUL...; Ahcene HAKEM; Mohamed-Nabil MEN...; Carmen TORRES
• Keywords: Carriage

This study aimed to characterize the fecal carriage of antimicrobial-resistant Escherichia coli isolates in healthy bovine Northern Algeria.Fecal samples 233 cows were collected and cultured on MacConkey agar.E. recovered, identified tested for antibiotic susceptibility by disk diffusion method.Screening extendedspectrum-betalactamase (ESBL)-production was performed double-disk synergy test characterization ESBL genes PCR sequencing.All typed phylogenetic groups multilocus-sequence-typing (MLST) analysis phylogroup B2 ESBL-producing isolates.The presence antimicrobial resistant analyzed collection E. integrons SXT-resistant isolates.Overall, 39.9% (89/223) at least one agent, 41.5% them showed multi-drug resistance (MDR).High rates detected tetracycline (32.3%), streptomycin (18.4%), sulphamethoxazole/ trimethoprim (15.7%) ampicillin (15.2%).Two identified: A/ST617/CTX-M-15 A/ST48/SHV-12.Sequence types ST95, ST998 ST145 among isolates.From 35 SXT R isolates, class-1 class-2 82.9% (29/35) 12.9% (1/35), respectively.Six gene-cassette-array structures variable region (dfrA1-aadA; dfrA12-aadA2, aadA1/2; dfrA12-orfF-aadA2-cml-sul3-linked dfrA17-aadA5) (dfrA1-sat2-aadA1).Our highlights potential dynamics animal farms.