A5070343943- Plant biochemistry and biosynthesis
- Insect-Plant Interactions and Control
- Plant and animal studies
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- Insect and Pesticide Research
- Plant Parasitism and Resistance
- Alcohol Consumption and Health Effects
- Lipid Membrane Structure and Behavior
- Gut microbiota and health
- Plant Reproductive Biology
- Folate and B Vitamins Research
- Biochemical and biochemical processes
- Plant Pathogens and Fungal Diseases
- Spectroscopy and Quantum Chemical Studies
- Plant Virus Research Studies
- Protein Structure and Dynamics
- Ecology and Vegetation Dynamics Studies
- Plant Molecular Biology Research
- Metabolism and Genetic Disorders
- Probiotics and Fermented Foods
- biodegradable polymer synthesis and properties
- Horticultural and Viticultural Research
- Drug Transport and Resistance Mechanisms
Michigan State University
2016-2025
University of Michigan
2010-2011
University of Arizona
2010
Plant Biotechnology Institute
2009
National Academies of Sciences, Engineering, and Medicine
2009
The University of Texas Southwestern Medical Center
2009
Southwestern Medical Center
2009
Health First
2009
ARC Centre of Excellence in Plant Energy Biology
2008
Jasmonate (JA) is a lipid-derived hormone that regulates diverse aspects of plant immunity and development. An amino acid-conjugated form JA, jasmonoyl–isoleucine (JA–Ile), stimulates binding the F-box protein coronatine-insensitive 1 (COI1) to, subsequent ubiquitin-dependent degradation of, jasmonate ZIM domain (JAZ) proteins repress transcription JA-responsive genes. The virulence factor coronatine (COR), which produced by pathogenic strains Pseudomonas syringae , suppresses host defense...
We identified a cis-prenyltransferase gene, neryl diphosphate synthase 1 (NDPS1), that is expressed in cultivated tomato (Solanum lycopersicum) cultivar M82 type VI glandular trichomes and encodes an enzyme catalyzes the formation of from isopentenyl dimethylallyl diphosphate. mRNA for terpene phellandrene (PHS1), was also these glands. It uses to produce beta-phellandrene as major product well variety other monoterpenes. The profile monoterpenes produced by PHS1 identical with found NDPS1...
Plant trichomes come in a variety of shapes, sizes and cellular composition. Some types, commonly called glandular trichomes, produce large amounts specialized (secondary) metabolites diverse classes. Trichomes are implicated adaptive processes, including defense against herbivores micro-organisms as well ion homeostasis. Because protrude from the epidermis can often be easily separated it harvested, mRNAs, proteins small molecules that they contain unusually accessible to analysis. This...
Abstract Compounds of the terpenoid class play numerous roles in interactions plants with their environment, such as attracting pollinators and defending plant against pests. We show here that genome cultivated tomato (Solanum lycopersicum) contains 44 terpene synthase (TPS) genes, including 29 are functional or potentially functional. Of these TPS 26 were expressed at least some organs tissues plant. The enzymatic functions eight proteins previously reported, we report specific vitro...
Summary The initial reactions of the phenylpropanoid pathway convert phenylalanine to p ‐coumaroyl CoA, a branch point metabolite from which many phenylpropanoids are made. Although second enzyme this pathway, cinnamic acid 4‐hydroxylase (C4H), is well characterized, mutant for gene encoding has not yet, our knowledge, been identified, presumably because knock‐out mutations in would have severe phenotypes. This work describes characterization an allelic series Arabidopsis reduced epidermal...
Highbush blueberry (Vaccinium corymbosum) has long been consumed for its unique flavor and composition of health-promoting phytonutrients. However, breeding efforts to improve fruit quality in have greatly hampered by the lack adequate genomic resources a limited understanding underlying genetics encoding key traits. The genome highbush particularly challenging assemble due, large part, polyploid nature size.
Abstract Jasmonic acid (JA) is a lipid-derived signal that regulates plant defense responses to biotic stress. Here, we report the characterization of JA-deficient mutant tomato (Lycopersicon esculentum) lacks local and systemic expression defensive proteinase inhibitors (PIs) in response wounding. Map-based cloning studies demonstrated this phenotype results from loss function an acyl-CoA oxidase (ACX1A) catalyzes first step peroxisomal β-oxidation stage JA biosynthesis. Recombinant ACX1A...
Abstract The biosynthesis of jasmonic acid (JA) in plant peroxisomes requires the action acyl-coenzyme A oxidase (ACX). Among five expressed members (ACX1–5) ACX gene family Arabidopsis (Arabidopsis thaliana), only ACX1 is known to serve a role JA production. Here, we used transgenic promoter-reporter lines show that highly mature and germinating pollen, stem epidermal cells, other tissues which jasmonate-signaled processes occur. Wound-induced accumulation was reduced mutant defective...
Glandular secreting trichomes of cultivated tomato (Solanum lycopersicum) and close relatives produce a variety structurally diverse volatile non-volatile specialized ('secondary') metabolites, including terpenes, flavonoids acyl sugars. A genetic screen is described here to profile leaf trichome surface metabolite extracts nearly isogenic chromosomal substitution lines covering the genome. These contain specific regions Solanum pennellii LA0716 genome in an otherwise 'wild-type' M82...
Abstract Glandular trichomes play important roles in protecting plants from biotic attack by producing defensive compounds. We investigated the metabolic profiles and transcriptomes to characterize differences between different glandular trichome types several domesticated wild Solanum species: lycopersicum (glandular 1, 6, 7), habrochaites (types 4, 6), pennellii 4 arcanum (type pimpinellifolium 6). Substantial chemical species are likely determined regulation of metabolism at levels....
Glandular secreting trichomes on the surface of tomato plants and many its relatives in Solanaceae produce a mixture O -acyl sugars that contribute to insect resistance. The majority acyl sucroses produced by cultivated ( Solanum lycopersicum ) contain three or four short chain aliphatic esters, tetra-acyl have an acetyl group as one chains. We previously reported overlapping S. × pennellii introgression lines (ILs) fail accumulate high levels acetylated sucroses. A survey annotated genes...
Structural and functional characterization of the extracellular domain human CaSR with bound Mg 2+ a tryptophan derivative.
Abstract Shotgun proteomics analysis allows hundreds of proteins to be identified and quantified from a single sample at relatively low cost. Extensive DNA sequence information is prerequisite for shotgun proteomics, it ideal have the organism being studied rather than related species or accessions. While this requirement has limited set organisms that are candidates approach, next generation sequencing technologies make feasible obtain deep coverage any organism. As part our studies...
Significance Throughout the course of human history, plant-derived natural products have been used in medicines, cooking, as pest control agents, and rituals cultural importance. Plants produce rapidly diversifying specialized metabolites protective agents to mediate interactions with beneficial organisms. In vitro reconstruction cultivated tomato insect acylsucrose biosynthetic network showed that four acyltransferase enzymes are sufficient full set naturally occurring compounds. This...
Glandular trichomes from tomato (Solanum lycopersicum) and other species in the Solanaceae produce secrete a mixture of O-acylsugars (aliphatic esters sucrose glucose) that contribute to insect defense. Despite their phylogenetic distribution diversity, relatively little is known about how these specialized metabolites are synthesized. Mass spectrometric profiling acylsugars S. lycopersicum x Solanum pennellii introgression lines identified chromosome 11 locus containing cluster BAHD...
Evolution of a cell specific invertase enzyme creates phenotypic diversity in wild tomato insecticidal metabolite production.
Glandular trichomes of cultivated tomato (Solanum lycopersicum) and many other species throughout the Solanaceae produce secrete mixtures sugar esters (acylsugars) on plant aerial surfaces. In wild tomato, these metabolites consist a backbone, typically glucose or sucrose, two to five acyl chains esterified various positions core. The aliphatic vary in length branching are transferred by series reactions catalyzed acylsugar acyltransferases. A phenotypic screen set S. lycopersicum M82 ×...
Flavonoids are ubiquitous plant aromatic specialized metabolites found in a variety of cell types and organs. Methylated flavonoids detected secreting glandular trichomes various Solanum species, including the cultivated tomato (Solanum lycopersicum). Inspection sequenced S. lycopersicum Heinz 1706 reference genome revealed close homolog habrochaites MOMT1 3'/5' myricetin O-methyltransferase gene, but this gene (Solyc06g083450) is missing first exon, raising question whether has distinct 3'...
Flowers of Tanacetum cinerariifolium produce a set compounds known collectively as pyrethrins, which are commercially important pesticides that strongly toxic to flying insects but not most vertebrates. A pyrethrin molecule is an ester consisting either trans-chrysanthemic acid or its modified form, pyrethric acid, and one three alcohols, jasmolone, pyrethrolone, cinerolone, appear be derived from jasmonic acid. Chrysanthemyl diphosphate synthase (CDS), the first enzyme involved in synthesis...
Summary In the natural pesticides known as pyrethrins, which are esters produced in flowers of Tanacetum cinerariifolium (Asteraceae), monoterpenoid acyl moiety is pyrethric acid or chrysanthemic acid. We show here that from chrysanthemol six steps catalyzed by four enzymes, first five occurring trichomes covering ovaries and last one inside ovary tissues. Three involve successive oxidation carbon 10 (C10) to a carboxylic group Tc CHH , cytochrome P450 oxidoreductase. Two other hydroxylated...
Repeating l- and d-chiral configurations determine polylactide (PLA) stereochemistry, which affects its thermal physicochemical properties, including degradation profiles. Clinically, of implanted PLA biomaterials promotes prolonged inflammation excessive fibrosis, but the role stereochemistry is unclear. Additionally, although varied stereochemistries causes differential immune responses in vivo, this observation has yet to be effectively modeled vitro. A bioenergetic model was applied...
Allene oxide synthase (AOS) is a cytochrome P-450 (CYP74A) that catalyzes the first step in conversion of 13-hydroperoxy linolenic acid to jasmonic and related signaling molecules plants. Here, we report molecular cloning characterization novel AOS-encoding cDNA (LeAOS3) from Lycopersicon esculentum whose predicted amino sequence classifies it as member CYP74C subfamily enzymes was hitherto not known include AOSs. Recombinant LeAOS3 expressed Escherichia coli showed spectral characteristics...