A5014168915- Fungal Infections and Studies
- Cancer Immunotherapy and Biomarkers
- Antifungal resistance and susceptibility
- Immune Cell Function and Interaction
- Hepatitis C virus research
- Hepatitis B Virus Studies
- CAR-T cell therapy research
- Plant-Microbe Interactions and Immunity
- Immunotherapy and Immune Responses
- Liver Disease Diagnosis and Treatment
- Melanoma and MAPK Pathways
- Chemokine receptors and signaling
- Diet, Metabolism, and Disease
- HIV/AIDS drug development and treatment
- Heme Oxygenase-1 and Carbon Monoxide
- Yeasts and Rust Fungi Studies
- DNA Repair Mechanisms
- Nail Diseases and Treatments
- RNA modifications and cancer
- T-cell and B-cell Immunology
- Adipose Tissue and Metabolism
- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
- Adipokines, Inflammation, and Metabolic Diseases
- Cancer-related Molecular Pathways
Saint Louis University
2015-2024
UCLouvain Saint-Louis Brussels
2018-2024
Institute for Learning Innovation
2022
Saint Louis University
2019
Brigham and Women's Hospital
2014
Harvard Stem Cell Institute
2014
Harvard University
2014
Broad Institute
2014
Confluence Life Sciences (United States)
2014
Center of Molecular Immunology (Cuba)
2008
Cryptococcus neoformans is a basidiomycetous yeast ubiquitous in the environment, model for fungal pathogenesis, and an opportunistic human pathogen of global importance. We have sequenced its approximately 20-megabase genome, which contains 6500 intron-rich gene structures encodes transcriptome abundant alternatively spliced antisense messages. The genome rich transposons, many cluster at candidate centromeric regions. presence these transposons may drive karyotype instability phenotypic...
Cryptococcus neoformans is an opportunistic fungal pathogen that causes cryptococcal meningoencephalitis, particularly in immunocompromised patients. The cell wall excellent target for antifungal therapies as it essential organelle provides structure and integrity, needed the localization or attachment of known virulence factors, including polysaccharide capsule, melanin, phospholipase, critical host-pathogen interactions. In C. neoformans, chitosan produced by enzymatic removal acetyl...
Chitin is an essential component of the cell wall many fungi. also can be enzymatically deacetylated to chitosan, a more flexible and soluble polymer. Cryptococcus neoformans fungal pathogen that causes cryptococcal meningoencephalitis, particularly in immunocompromised patients. In this work, we show both chitin chitosan are present vegetatively growing C. yeast cells levels rise dramatically as grow higher density liquid culture. has eight putative synthases, strains with any one synthase...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTKinetic partitioning between the exonuclease and polymerase sites in DNA error correctionMaureen J. Donlin, Smita S. Patel, Kenneth A. JohnsonCite this: Biochemistry 1991, 30, 2, 538–546Publication Date (Print):January 15, 1991Publication History Published online1 May 2002Published inissue 15 January 1991https://pubs.acs.org/doi/10.1021/bi00216a031https://doi.org/10.1021/bi00216a031research-articleACS PublicationsRequest reuse permissionsArticle...
Abstract A genome browser is software that allows users to visualize DNA, protein, or other sequence features within the context of a reference sequence, such as chromosome contig. The Generic Genome Browser (GBrowse) an open‐source developed part Model Organism Database project (Stein et al., 2002). GBrowse can be configured display genomic for any organism and used model organisms Drosophila melanogaster (Grumbling Strelets, 2006) Caenorhabditis elegans (Schwarz 2006), among others....
Abstract Neoplastic cells rely on the tumor microenvironment (TME) for survival and progression factors. Indeed, senescent cancer-associated fibroblasts (CAF) express factors that promote tumorigenesis are collectively referred to as senescence-associated secretory phenotype (SASP). Despite their importance in tumorigenesis, mechanisms control TME-derived factor expression remain poorly understood. Here, we address a key unanswered question: how SASP is sustained CAFs. We find...
Cell wall biogenesis and integrity are crucial for fungal growth, pathogenesis survival, attractive targets antifungal therapy. In this study, we identify, delete analyse mutant strains 10 genes involved in the PKC1 signal transduction pathway its regulation Cryptococcus neoformans. The kinases Bck1 Mkk2 critical maintaining integrity, deletion of each these causes severe phenotypes different from other. stark contrast to results seen Saccharomyces cerevisiae, a LRG1 has repercussions cell,...
ABSTRACT Pegylated alpha interferon and ribavirin therapy for hepatitis C virus (HCV) genotype 1 infection fails half of Caucasian American patients (CA) more often African Americans (AA). The reasons these low response rates are unknown. HCV is highly genetically variable, but it unknown how this variability affects to therapy. To assess effects viral diversity on therapy, the complete pretreatment open reading frame was sequenced using samples from 94 participants in Virahep-C study....
Summary The polysaccharide β‐1,6‐glucan is a major component of the cell wall Cryptococcus neoformans , but its function has not been investigated in this fungal pathogen. We have identified and characterized seven genes, belonging to KRE family, which are putatively involved synthesis. H99 deletion mutants kre5 Δ kre6 skn1 contained less β‐1,6‐glucan, grew slowly with an aberrant morphology, were highly sensitive environmental chemical stress avirulent mouse inhalation model infection....
Cryptococcus neoformans is unique among fungal pathogens that cause disease in a mammalian host, as it secretes polysaccharide capsule hinders recognition by the host to facilitate its survival and proliferation. Even though causes serious infections immunocompromised hosts, reports of infection hosts are immunocompetent on rise. The cell wall pathogen, synthesis, composition, pathways remodelling attractive therapeutic targets for development fungicides. Chitosan, C. one such target,...
Hepatitis C virus (HCV) is a common RNA that causes hepatitis and liver cancer. Infection treated with IFN-α ribavirin, but this expensive physically demanding therapy fails in half of patients. The genomic sequences independent HCV isolates differ by approximately 10%, the effects variation on response to are unknown. To address question, we analyzed amino acid covariance within full viral coding region pretherapy from 94 participants Viral Resistance Antiviral Therapy Chronic (Virahep-C)...
The ability of the opportunistic fungal pathogen Cryptococcus neoformans to resist oxidative stress is one its most important virulence related traits. To cope with deleterious effect cellular damage caused by burst inside macrophages, C. has developed multilayered redundant molecular responses neutralize stress, repair and eventually grow hostile environment phagosome. We used microarray analysis cells treated hydrogen peroxide (H2O2) at multiple time points in a nutrient defined medium...
ABSTRACT The ability of the fungal pathogen Cryptococcus neoformans to evade mammalian innate immune response and cause disease is partially due its respond survive nitrosative stress. In this study, we use proteomic genomic approaches elucidate C. nitric oxide This stress involves both transcriptional, translational, posttranslational regulation. Proteomic analyses reveal changes in expression genes. addition, genes involved cell wall organization, respiration, signal transduction,...
Objectives. We examined the effectiveness of tailored calendars in increasing childhood immunization rates. Methods. Parents babies aged birth to 1 year (n = 321) received individually promoting from 2 urban public health centers. For each baby, an age- and sex-matched control was selected same center. Immunization status tracked through age 24 months. Results. A higher proportion intervention than were up date at end a 9-month enrollment period (82% vs 65%, P < .001) months (66% 47%,...
Pegylated interferon plus ribavirin therapy for hepatitis C virus (HCV) fails in approximately half of genotype 1 patients. Treatment failure occurs either by nonresponse (minimal declines viral titer) or relapse (robust initial responses followed rebounds titers during after therapy). HCV is highly variable genetically. To determine if genetic differences contribute to the difference between response and relapse, we examined inter-patient diversity mutation pattern full open reading frame...
ABSTRACT Cryptococcus neoformans is a fungal pathogen of immunocompromised people that causes fatal meningitis. The cell wall essential to viability and pathogenesis C. , biosynthesis repair the primarily controlled by integrity (CWI) signaling pathway. Previous work has shown deletion genes encoding four major kinases in CWI pathway, namely, PKC1 BCK1 MKK2 MPK1 results severe phenotypes, sensitivity variety stressors, for Mpk1, reduced virulence mouse model. Here, we examined global...
The intrinsic resistance of Cryptococcus neoformans to the cell wall inhibitor caspofungin limits available therapies for treating cryptococcal infections. We screened a collection more than 4,000 gene deletion strains altered sensitivity identify biological processes that could be targeted render susceptible caspofungin.
Abstract The regulatory transposon of the Mutator system transposable elements in maize is MuDR. MuDR produce two transcripts, from genes mudrA and mudrB, encoding proteins MURA MURB, respectively. Like many other transposons, often undergo deletions, usually internal sequences. Analysis a deletion that restricted to region MURB demonstrates this gene not required cause excisions reporter element, although it may be for transposition or suppression suppressible alleles. Conversely,...
Cryptococcus neoformans is a pathogen that common in immunosuppressed patients. It can be treated with amphotericin B and fluconazole, but the mortality rate remains 15 to 30%. Thus, novel more effective anticryptococcal therapies are needed. The troponoids based on natural products isolated from western red cedar, have broad range of antimicrobial activities. Extracts cedar inhibit growth several fungal species, neither extracts nor troponoid derivatives been tested against C. We screened...
Coinhibitory receptor blockade is a promising strategy to boost T-cell immunity against variety of human cancers. However, many patients still do not benefit from this treatment, and responders often experience immune-related toxicities. These issues highlight the need for advanced mechanistic understanding improve patient outcomes uncover clinically relevant biomarkers treatment efficacy. T-cell-intrinsic signaling pathways engaged during checkpoint are well defined, particularly...
Novel antibiotics are urgently needed. The troponoids [tropones, tropolones, and α-hydroxytropolones (α-HT)] can have anti-bacterial activity. We synthesized or purchased 92 evaluated their antibacterial activities against Staphylococcus aureus, Escherichia coli, Acinetobacter baumannii, Pseudomonas aeruginosa. Preliminary hits were assessed for minimum inhibitory concentrations (MIC80) cytotoxicity (CC50) human hepatoma cells. Sixteen inhibited S. aureus/E. coli/A. baumannii growth by ≥80%...