A5033918180- Neuropeptides and Animal Physiology
- Pain Mechanisms and Treatments
- Migraine and Headache Studies
- Receptor Mechanisms and Signaling
- Hydrogen's biological and therapeutic effects
- Growth Hormone and Insulin-like Growth Factors
- Redox biology and oxidative stress
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Pain Management and Treatment
- bioluminescence and chemiluminescence research
- Neuroendocrine regulation and behavior
- Advanced Fluorescence Microscopy Techniques
- Plant-based Medicinal Research
- Nicotinic Acetylcholine Receptors Study
- Biochemical effects in animals
- Genomics, phytochemicals, and oxidative stress
- Cerebrovascular and Carotid Artery Diseases
- Protein Hydrolysis and Bioactive Peptides
- Cancer, Stress, Anesthesia, and Immune Response
- Traumatic Brain Injury and Neurovascular Disturbances
- Neurological Disorders and Treatments
- Salivary Gland Disorders and Functions
- Intracranial Aneurysms: Treatment and Complications
- Neurological Disease Mechanisms and Treatments
- Stress Responses and Cortisol
University of Pecs
2018-2025
Research Network (United States)
2023
Abstract Background Recent data suggest that gene expression profiles of peripheral white blood cells can reflect changes in the brain. We aimed to analyze transcriptome mononuclear (PBMC) and plasma metabolite levels migraineurs a self-controlled manner during between attacks. Methods Twenty-four patients with migraine were recruited samples collected headache-free (interictal) period headache (ictal) investigate disease- headache-specific alterations. Control from 13 age- sex-matched...
Orofacial pain and headache disorders are among the most debilitating conditions. While pathophysiological basis of these may be diverse, it is generally accepted that a common mechanism behind arising sensitization extra- intracranial trigeminal primary afferents. In present study we investigated gene expression changes in ganglia (TRG), nucleus caudalis (TNC) peripheral blood mononuclear cells (PBMC) evoked by Complete Freund's Adjuvant (CFA)-induced orofacial inflammation rats, as model...
Migraine is a primary headache with genetic susceptibility, but the pathophysiological mechanisms are poorly understood, and it remains an unmet medical need. Earlier we demonstrated significant differences in transcriptome of migraineurs' PBMCs (peripheral blood mononuclear cells), suggesting role neuroinflammation mitochondrial dysfunctions. Post-transcriptional gene expression regulated by miRNA (microRNA), group short non-coding RNAs that emerging biomarkers, drug targets, or drugs....
Abstract Background Trigeminal activation and sensitisation involved in chronic inflammatory orofacial pain share several similarities with headaches, including migraine. Therefore, understanding the pathophysiological mechanisms is important to determine novel therapies, which animal models are crucial. Here we aimed identify key mediators, networks using unbiased multi-omic approaches a rat model. Methods Complete Freund’s Adjuvant (CFA, 50 µl, 1 mg/mL) was injected into right whisker pad...
A large percentage of primary sensory neurons in the trigeminal ganglia (TG) contain neuropeptides such as tachykinins or calcitonin gene-related peptide. Neuropeptides released from central terminals afferents sensitize secondary nociceptive nucleus caudalis (TNC), but also activate glial cells contributing to neuroinflammation and consequent sensitization chronic orofacial pain migraine. In present study, we investigated newest member tachykinin family, hemokinin-1 (HK-1) encoded by Tac4...
Somatostatin receptor subtype 4 (SST4) has been shown to mediate analgesic, antidepressant and anti-inflammatory functions without endocrine actions; therefore, it is proposed be a novel target for drug development. To overcome the species differences of SST4 expression function between humans mice, we generated an humanized mouse line serve as translational animal model preclinical research. A transposon vector containing hSSTR4 reporter gene construct driven by regulatory elements were...
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a broadly expressed neuropeptide which has diverse effects in both the peripheral and central nervous systems. While its neuroprotective have been shown variety of disease models, animal human data support role PACAP migraine generation. Both truncated derivative PACAP(6-38) increased calcium influx rat trigeminal ganglia (TG) primary sensory neurons most experimental settings. PACAP(6-38), however, described as an antagonist for...
It has previously been shown that the peptide (34Pro,35Phe)CGRP27-37 is a potent calcitonin gene-related peptide, CGRP receptor antagonist, and in this project we aimed to improve antagonist potency through structural modification of truncated C-terminal peptides.Six analogues were synthesized anti-CGRP activity confirmed using both vitro vivo studies.A 10 amino acid-containing VPTDVGPFAF-NH2 (P006) was identified as key candidate take forward for evaluation, where it be an effective after...
The tachykinin hemokinin-1 (HK-1) is involved in immunological processes, inflammation, and pain. Although the neurokinin 1 receptor (NK1R) described as its main target, several effects are mediated by currently unidentified receptor(s). role of HK-1 pain controversial, depending on involvement peripheral central sensitization mechanisms different models. We earlier showed ability to activate trigeminovascular system, but need be clarified. Therefore, this study, we investigated HK-1-induced...
Chronic orofacial pain disorders are common debilitating conditions, affecting the trigeminal system. Its underlying pathophysiological mechanisms still unclear and therapy is often unsatisfactory, therefore, preclinical models crucial to identify key mediators novel treatment options. Complete Freund’s adjuvant (CFA)-induced inflammatory allodynia/hyperalgesia commonly used in rodents, but it has not been validated with currently drugs. Here we tested effects of analgesic/antiepileptic...
Intraoperative stress is common to patients undergoing carotid endarterectomy (CEA); thus, impaired oxygen and metabolic balance may appear. In this study, we aimed identify new markers of intraoperative cerebral ischemia, with predictive value on postoperative complications during CEA, performed in regional anesthesia. A total 54 significant stenosis were recruited submitted CEA. Jugular arterial blood samples taken four times operation, measure the jugulo-arterial carbon dioxide partial...