Abstract Background Recently, plasma miRNAs have been reported as biomarkers for various diseases. However, the knowledge on association of with ischemic stroke is still lacking. In this study, we investigated whether concentrations miR-30a, miR-126 and let-7b may be in humans. Methods One hundred ninety seven patients were recruited their blood samples collected at 24 h, 1 week, 4 weeks, weeks 48 after symptoms onset, fifty healthy volunteers selected control. Levels miRNA quantified by...

Fusarium wilt of banana (Musa spp.) caused by the fungal pathogen oxysporum f. sp. cubense (Foc) is a typical soilborne disease, that severely devastates industry worldwide, and soil microbial diversity closely related to spread wilt. To understand relationship between species wilt, it important wilt-diseased disease-free soils from fields. Based on sequencing analysis bacterial 16S rRNA genes internal transcribed spacer (ITS) sequences, Foc abundance, or richness were higher in diseased...

Banana wilt disease, caused by the fungal pathogen Fusarium oxysporum f. sp. cubense 4 (Foc4), is regarded as one of most devastating diseases worldwide. Cavendish cultivar 'Yueyoukang 1' was shown to have significantly lower disease severity and incidence compared with susceptible 'Brazilian' in greenhouse field trials. De novo sequencing technology previously performed investigate defense mechanism middle resistant 'Nongke No banana, but not highly 1'. To gain more insights into resistance...

A heavy metal-resistant and dimethyl disulfide-producing bacterial strain, designated 6NM-7T, was isolated from wolfram mine tailing, Dayu County, Jiangxi Province, PR China. Strain 6NM-7T aerobic, Gram-stain-negative motile by means of a single polar flagellum. Phylogenetic analysis, based on 16S rRNA gene sequences, showed that strain affiliated with the genus Massilia closely related to norwichensis LMG 28164T (98.8 % sequence similarity), kyonggiensis KACC 17471T (98.4 %), niastensis...

As an irreversible and complex degenerative physiological process, the treatment for aging seems strategically necessary, polysaccharides play important roles against owing to their abundant bioactivities. In this paper, antioxidant anti-aging activities of Flammulina velutipes (FPS) its sulfated FPS (SFPS) on d-galactose-induced mice were investigated. The in vitro demonstrated that SFPS had strong reducing power superior scavenging effects 2, 2-diphenylpicrylhydrazyl (DPPH), hydroxyl...

Fusarium wilt of banana is one the most destructive diseases in world. This disease has caused heavy losses major production areas. Except for molecular breeding methods based on plant defense mechanisms, effective to control are still lacking. Dynamic changes mechanisms between susceptible, moderately resistant, and highly resistant oxysporum f. sp. cubense tropical race 4 (Foc4) at protein level remain unknown. research reports proteomic profile three cultivars response Foc4...

A yellow-pigmented bacterial strain, designated 9NM-10T, was isolated from an abandoned lead-zinc mine in Meizhou, Guangdong Province, China. Cells were strictly aerobic, Gram-stain-negative and motile with a polar monotrichous flagellum. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain 9NM-10T belongs to the genus Sphingomonas most closely related yantingensis JCM 19201T japonica 15438T. DNA-DNA relatedness values between these two type strains 43.6±1.3 35.4±0.9...

Dilated cardiomyopathy (DCM) induced chronic heart failure is a life-threatening disease worldwide. Long non-coding RNAs (lncRNAs) are potential new therapeutic targets and may provide pathophysiological mechanisms for development of DCM.Microarray assays in 14 DCM 10 control human samples identified 313 significantly differentially expressed lncRNAs. SiRNAs were used to explore the function specific lncRNAs (RP11-544D21.2 XLOC_014288) cardiac myocytes, fibroblasts microvascular endothelial...

MicroRNAs (miRNAs) are small non-coding RNAs that regulate protein-coding gene expression primarily found in plants and animals. Fungi produce microRNA-like (milRNAs) structurally similar to miRNAs functionally important various biological processes. The fungus Fusarium oxysporum f. sp. cubense ( Foc ) is the causal agent of Banana vascular wilt threatens global banana production. It remains uncharacterized about biosynthesis functions milRNAs . In this study, we investigated function...

Aberrant expression of heparanase (Hpa) is associated with apoor prognosis in ovarian and cervical cancer patients. Inhibitors Hpa can prevent the growth metastasis malignant tumor cells, suramin may be such a compound that has strong anti-proliferative effects on several kinds cells. We have therefore tested whether inhibiting effect cells due to downregulation expression.Suramin at 300-600 μg/ml significantly inhibited HO-8910 PM HeLa cell 24 h, both time-dependent dose-dependent manner,...

Banana Fusarium wilt, caused by oxysporum f. sp. cubense tropical race 4 (Foc TR4), is a major disease of banana plants worldwide. Effector proteins play critical roles in banana-Foc TR4 interaction. Our previous studies highlighted ribonuclease protein belonging to the T2 family (named as FocRnt2) Foc secretome, which was predicted be an effector. However, its biological function infection still unclear. Herein, we observed significant expression FocRnt2 during early stage fungal planta. A...

Recent studies suggest that plant pectin methylesterases (PMEs) are directly involved in defence besides their roles development. However, the molecular mechanisms of PME action on pectins not well understood. In order to understand how PMEs modify during banana (Musa spp.)-Fusarium interaction, expression and enzyme activities two cultivars, highly resistant or susceptible Fusarium, were compared with each other. Furthermore, spatial distribution effect methylesterification 10 individual...

Fusarium oxysporum f. sp. cubense, the causative agent of Panama disease, is classified into three races: Foc1, Foc2, and Foc4. However, histological characteristics, accumulation fusaric acid (FA), resistant gene expression in banana infected with different races remain unclear. In this study, we compared infection processes, FA contents, levels a Cavendish cultivar (Musa AAA Brazilian) inoculated Foc1 Results showed that Foc4 can rapidly extend from roots to leaves, whereas expands slowly...

Long non-coding RNAs (lncRNAs) participate in the pathogenesis of cardiovascular diseases. However, whether circulating lncRNAs serve as dilated cardiomyopathy (DCM) biomarkers remains unclear.Totally, 266 controls and 818 patients were enrolled. First, microarray-based lncRNA profiling was performed 10 normal with DCM. Second, top 20 differentially expressed validated by real-time qPCR 64 DCM patients. Moreover, sequencing three human heart-derived cell types, correlation between levels...

The present work mainly describes the preparation of acetylated mycelia polysaccharides (AMPS) from Pleurotus djamor and investigates antioxidant anti-aging effects in d-galactose-induced aging mice. optimized procedure indicates acetyl substitution degree AMPS is 0.54 ± 0.04 under conditions a reaction time 56 h, temperature 37 °C, 4 mL added acetic anhydride. vitro analysis vivo animal experiments indicate that could alleviate properties by scavenging radicals, elevating enzyme activities,...

Abstract Background Banana Fusarium wilt is a devastating disease of bananas caused by oxysporum f. sp. cubense (Foc) and serious threat to the global banana industry. Knowledge pathogenic molecular mechanism interaction between host Foc limited. Results In this study, we confirmed changes gene expression pathways in Cavendish variety ‘Brazilian’ during early infection with Foc1 Foc4 comparative transcriptomics analysis. 1862 226 differentially expressed genes (DEGs) were identified roots at...

Vitiligo is a common skin disease with complex pathogenesis, and so far, no effective treatment available. Lycium barbarum L. (Solanaceae) polysaccharide (LBP), the main active ingredient of goji berries, has been demonstrated to protect keratinocytes fibroblasts against oxidative stress.This study explored effects mechanism LBP on monobenzone-induced vitiligo in mice.C57BL/6 mice were randomly divided into five groups (n = 6): negative control that received vaseline, model group induced by...

// Jiahui Fan 1 , Huaping Li Xiang Nie Zhongwei Yin Yanru Zhao Chen Dao Wen Wang Division of Cardiology, Department Internal Medicine, Tongji Hospital, Medical College, Huazhong University Science and Technology, Wuhan 430030, China Correspondence to: Chen, email: chenchen@tjh.tjmu.edu.cn Wang, dwwang@tjh.tjmu.edu.cn Keywords: miR-30c-5p, NAFLD, hepatic steatosis, FASN, db/db Received: November 11, 2016 Accepted: January 02, 2017 Published: 09, ABSTRACT Approximately 15–40% the general adult...

Plant cell walls, which are mainly composed of pectin, play important roles in plant defence responses to pathogens. Pectin is synthesised a highly esterified form and then de-esterified by pectin methylesterases (PMEs). Because this, PMEs directly involved defence. However, the molecular mechanisms their interactions with pectins remain unclear. In this study, we compared expression level enzyme activities banana Cavendish cultivar (Musa AAA 'Brazilian') inoculated Fusarium oxysporum f. sp....

Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) is an important soilborne fungal pathogen that causes the most devastating banana disease. Effectors secreted by microbes contribute to virulence on host plants in plant-microbe interactions. However, functions of Foc TR4 effectors remain largely unexplored. In this study, we characterized a novel cupin_1 domain-containing protein (FoCupin1) from TR4. Sequence analysis indicated homologous proteins FoCupin1 phytopathogenic fungi...

Fusarium oxysporum f. sp. cubense (Foc) is a devastating plant pathogen that caused great financial loss in the banana's source area. Metatranscriptomic analysis was used to determine diversity of mycoviruses 246 isolates F. cubense. Partial or nearly complete genomes 20 were obtained by BLASTp RNA sequences using NCBI database. These viruses grouped into five distinct lineages, namely Botourmiaviridae, Endornaviridae, Mitoviridae, Mymonaviridae, Partitiviridae, and two non-classified...

Three aerobic, pink-pigmented, Gram-negative, motile and rod-shaped bacterial strains, designated SD21 T , SI9 SB2 were isolated from the phyllosphere of healthy litchis collected three main producing sites Guangdong Province, PR China. The 16S rRNA gene analysis showed that strains belonged to genus Methylobacterium ( Mtb .) with highest similarity Mtb. komagatae DSM 19563 (98.7%) phyllosphaerae CBMB27 (99.8%), respectively, while strain Methylorubrum Mtr Mtr. suomiense 14458 (98.6%)....

Fusarium wilt caused by oxysporum f. sp. cubense tropical race 4 (Foc TR4) is the most destructive disease of banana. Effectors play a crucial role in Foc TR4–banana interaction; however, only few effectors have been functionally characterized. Our previous secretome studies on TR4 highlighted an uncharacterized protein without any conserved domains (named FoUpe9), which was predicted to be candidate effector. Herein, bioinformatics analysis showed that FoUpe9 highly among species. induced...