The N 6-methyladenosine (m6A) pathway has been widely described as a viral regulatory mechanism in animals. We previously reported that the capsid protein (CP) of alfalfa mosaic virus (AMV) interacts with Arabidopsis m6A demethylase ALKBH9B regulating abundance on RNAs (vRNAs) and systemic invasion floral stems. Here, we analyze involvement other ALKBH9 proteins AMV infection carry out detailed evaluation restraint observed alkbh9b mutant plants. Thus, via titer quantification experiments...

Nanoparticles are able to interact with biomolecules, creating functional nanosystems for transportation within in vivo cells, and leading the study of their potential applications field plant biotechnology. Therefore, aim this research was determine growth rooting effect functionalized (SWCNTs-COOH) non-functionalized nanoparticles iron residue inner particles (SWCNTs-Fe) blackberry (Rubus adenotrichos) vitro plants. Two types SWCNTs were used, both them characterized a solid sample through...

Molecular markers provide a useful method for genotype characterization and allow high precision determination of the genetic relationship between cultivars varieties. A system based on DNA sequences—which is known as barcoding—will choose one or several standard loci which can be sequenced compared to differentiate species. In this research, ITS, matK, trnH-psbA sequences were evaluated molecular identification seven F. carica genotypes, generating complete first two loci, but unable...

Nowadays, it has become urgently necessary to change the traditional methods of synthesis metallic nanoparticles, and start using safer more eco-friendly approaches.In present work a green silver nanoparticles was carried out mint leaf extract (Mentha piperita) as reducing stabilizing agent.Silver were synthesized 1 mM nitrate solution varying volume (150, 250, 350 L), at temperature 30 C for 24 h.The formation monitored by UV-Visible spectrophotometer which showed absorption peaks ranging...

N 6 -methyladenosine (m A) modification is a dynamically regulated RNA that impacts many cellular processes and pathways. This epitranscriptomic methylation relies on the participation of methyltransferases (referred to as “writers”) demethylases “erasers”), respectively. We previously demonstrated Arabidopsis thaliana protein at ALKBH9B showed m A-demethylase activity interacted with coat (CP) alfalfa mosaic virus (AMV), causing profound impact viral infection cycle. To dissect functional...

We have previously reported the presence of m6A in AMV (Alfamovirus, Bromoviridae) genome. Interestingly, two these putative m6A-sites are hairpin (hp) structures 3'UTR viral RNA3. One site (2012AAACU2016) is loop hpB, within coat protein binding 1 (CPB1), while other (1900UGACC1904) lower stem hpE, a associated with negative-strand RNA synthesis. In this work, we performed vivo experiments to assess role regions, containing cycle, by introducing compensatory point mutations interfere or...

Trichoderma species are non-pathogenic microorganisms that protect against fungal diseases and contribute to increased crop yields. However, not all have the same effects on or a pathogen, whereby characterization identification of strains at level is first step in use microorganism. The aim this study was – five isolated from soil samples obtained garlic onion fields located Costa Rica, through analysis ITS1, 5.8S, ITS2 ribosomal RNA regions; as well determination their individual...

Blackberries are fruits produced worldwide, with 25 % of their production centered in Mexico, Central and South America. Tropical highland blackberry is a fruit that can potentially enhance human health, due to high content phenolic compounds, which include anthocyanins, acids, tannins (gallotannins elagitannins) flavonoids. Therefore, the overall aim this study development callus induction protocol, establishment cell suspensions (Rubus adenotrichos Schltdl.) analysis through optical...

RESUMEN El membrillo (Cydonia oblonga) es un frutal no tradicional en Costa Rica que presenta propiedades médicas y nutricionales, sin embargo la lentitud del crecimiento enraizamiento dificulta obtener poblaciones homogéneas mediante técnicas convencionales. Es por esta razón investigación tuvo como objetivo producción de material vegetal uniforme tiempos reducidos empleando sistemas inmersión temporal (RITA ®). Se utilizó referencia medio semisólido MS, suplementado con 0,1 mg L-1 ANA; 0,3...

<p class="p1">El tomate de árbol (<em>Cyphomandra betacea</em>) es un frutal importancia comercial en países como Bolivia, Colombia, Ecuador y Perú, donde se consume fruta fresca procesada. Tradicionalmente, propagado por semillas estacas, enfrenta problemas heterogeneidad calidad material siembra. </p><p class="p1">En esta investigación desarroll<em>ó </em>un protocolo para la micropropagación <em>in vitro </em>del criollo del fenotipo...

Premise of research. Many plant species produce propagules different shapes and sizes on the same or within an inflorescence. These differences influence seed dispersal germination seedling growth. We investigated relationship between flooding regime, time fruiting season, position fruit coloration, abortion, predation in Sesbania emerus.Methodology. studied production seeds colors fruits S. emerus Palo Verde National Park, Costa Rica, during months maturation dispersal. sampled natural...

Over the last decade, high-end fruits such as berries, have faced impact of Drosophila suzukii (Matsumura, 1931) (Diptera: Drosophilidae) across Asia, Europe, and Americas. Yet, none countries from Central America has officially reported this pest. We carried out surveys in blackberry fields Costa Rica. placed a proprietary mixture liquid attractants into red-cup traps with lateral holes, it been widely demonstrated best to capture D. suzukii. Subsequently, we collected both adults larvae...

El presente estudio se enfocó en investigar la presencia del virus acucharamiento de hoja tomate (TYLCV) plantas el cantón Sarchí, Costa Rica. Se recolectaron muestras con síntomas enfermedad, así como una muestra sin síntomas, y realizaron análisis moleculares microscópicos para detectar caracterizar virus. demostró TYLCV las analizadas mediante PCR, comparando otras secuencias un BLAST árbol filogenético. Además, observaron diferencias gravedad los entre expuestas al sol a sombra. En por...

Seagrasses are a polyphyletic group of marine flowering plants that play crucial roles in nearshore ecology, yet their interactions with viruses remain largely unexplored. This study presents the construction and characterization an infectious cDNA clone potexvirus turtle grass virus X (TGVX). The complete genome this positive-sense single-stranded RNA was amplified from field samples

Abstract Sweet potato ( Ipomoea batatas ) is a crucial crop for food security and economic stability in many regions, including Costa Rica. This study aimed to assess the prevalence genetic diversity of two major viruses associated with sweet virus disease (SPVD) Rica: feathery mottle (SPFMV) chlorotic stunt (SPCSV). A total 50 leaf samples displaying typical viral symptoms were collected from potato‐growing regions. Using multiplex reverse transcription (RT)‐PCR, SPFMV was detected 82%...

Long terminal repeats (LTR) retrotransposons are transposable elements (TEs) representing major components of most plant genomes. The fixation additional conserved protein domains in their genomes is considered a rare event the course evolution. Such changes can bring novel functions and increase fitness by playing role regulation replicative cycle or affecting integration landscape so that detection new turn reveal important aspects host-TE interactions. We have mined angiosperm for...

<p>El objetivo de la presente investigación fue evaluar el uso del espaciador plastídico <em>trnH-psbA </em>y secuencia codificante <em>matK </em>como posibles marcadores para caracterización molecular dos accesiones membrillo (<em>C. oblonga</em>). Estas provinieron Argentina y Costa Rica. El trabajo se llevó a cabo en Centro Investigación Biotecnología Instituto Tecnológico Rica (ITCR), durante año 2014. Los productos amplificados fueron analizados...