Ronald J. Overmars
A5063790761- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- SARS-CoV-2 and COVID-19 Research
- Immune Response and Inflammation
- Animal Virus Infections Studies
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- HIV-related health complications and treatments
- Immune responses and vaccinations
- T-cell and B-cell Immunology
- Viral gastroenteritis research and epidemiology
- CRISPR and Genetic Engineering
- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- Immune Cell Function and Interaction
- Mosquito-borne diseases and control
- Immunotherapy and Immune Responses
- interferon and immune responses
Erasmus MC
2018-2023
Erasmus University Rotterdam
2020-2023
University of Amsterdam
2016-2017
Amsterdam UMC Location University of Amsterdam
2016-2017
Institute of Infection and Immunity
2017
10 days after the first reported case of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in Netherlands (on Feb 27, 2020), 55 (4%) 1497 health-care workers nine hospitals located south had tested positive for SARS-CoV-2 RNA. We aimed to gain insight possible sources workers.We did a cross-sectional study at three Netherlands. screened participating infection, based on clinical symptoms (fever or mild symptoms) before screening. obtained epidemiological data through...
Abstract Dengue virus (DENV) causes 400 million infections annually and is one of several viruses that can cause viral hemorrhagic fever, which characterized by uncontrolled immune activation resulting in high fever internal bleeding. Although the underlying mechanisms are unknown, massive cytokine secretion thought to be involved. Dendritic cells (DCs) main target DENV, we investigated their role DENV-induced production adaptive responses. DENV infection induced DC maturation IL-1β, IL-6,...
Follicular T helper cells (TFH) are fundamental in orchestrating effective antibody-mediated responses critical for immunity against viral infections and vaccines. However, it is unclear how virus infection leads to TFH induction. We here show that dengue (DENV) of human dendritic (DCs) drives formation via crosstalk RIG-I-like receptor (RLR) RIG-I MDA5 with type I Interferon (IFN) signaling. DENV RLR-dependent IKKε activation, which phosphorylates IFNα/β receptor-induced STAT1 drive IL-27...
Abstract SARS-CoV-2 is a novel coronavirus that has rapidly spread across the globe. In Netherlands, first case of been notified on 27 th February. Here, we describe three weeks outbreak in which started with several different introductory events from Italy, Austria, Germany and France followed by local amplification in, later also, outside South Netherlands. The timely generation whole genome sequences combined epidemiological investigations facilitated early decision making an attempt to...
Reactivation of the latent HIV-1 reservoir is a first step toward triggering decay. Here, we investigated impact BAF complex inhibitor pyrimethamine on people living with (PLWH). Twenty-eight PLWH suppressive antiretroviral therapy were randomized (1:1:1:1 ratio) to receive pyrimethamine, valproic acid, both, or no intervention for 14 days. The primary end point was change in cell-associated unspliced (CA US) RNA at days 0 and 14. We observed rapid, modest, significant increase response...
An amendment to this paper has been published and can be accessed via a link at the top of paper.
Substantial efforts to eliminate or reduce latent HIV-1 reservoirs are underway in clinical trials and have created a critical demand for sensitive, accurate, reproducible tools evaluate the efficacy of these strategies. Alternative reservoir quantification assays been developed circumvent limitations quantitative viral outgrowth assay. One such assay is tat/rev induced limiting dilution (TILDA), which measures frequency CD4+ T cells harboring inducible provirus. We modified...
We report a case of incomplete HIV-1 suppression on dolutegravir, lamivudine, and abacavir single-tablet regimen with the emergence H51Y G118R integrase resistance mutations. Integrase sequencing was performed retrospectively by Sanger next-generation sequencing. Rates decline mutations were calculated using data. Dolutegravir plasma concentrations measured ultra-performance liquid chromatography-tandem mass spectrometry. The effects infectivity, fitness, susceptibility to dolutegravir...
Abstract Ten days after the first reported case of SARS-CoV-2 infection in Netherlands, 3.9% healthcare workers (HCWs) nine hospitals located South Netherlands tested positive for RNA. The extent nosocomial transmission that contributed to HCW infections was unknown. We combined epidemiological data, collected by means structured interviews HCWs, with whole genome sequencing (WGS) clinical samples from HCWs and patients three participated screening, perform an in-depth analysis sources modes...
Although some individuals with HIV-2 develop severe immunodeficiency and AIDS-related complications, most may never progress to AIDS. Replication-competent isolated from asymptomatic long-term non-progressors (controllers) have lower replication rates than viruses who AIDS (progressors). To investigate potential retroviral factors that correlate disease progression in HIV-2, we sequenced the near full-length genomes of replication-competent previously outgrown controllers progressors used...
Abstract A major barrier towards HIV-1 cure is the presence of a replication-competent latent reservoir that, upon treatment cessation, can spark viral rebound leading to disease progression. Pharmacological reactivation with Latency reversing agents (LRAs) first step toward triggering decay. Inhibitors BAF-complex, key repressor transcription were identified act as LRAs, and enhanced effect other LRAs such histone deacetylase inhibitors ex-vivo. We repurposed licensed drug pyrimethamine...