A5043761055- Analytical Chemistry and Chromatography
- Mass Spectrometry Techniques and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Metabolomics and Mass Spectrometry Studies
- Spectroscopy and Laser Applications
- Advanced Proteomics Techniques and Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Spectroscopy Techniques in Biomedical and Chemical Research
- Trace Elements in Health
- Electrochemical sensors and biosensors
- Advanced Chemical Sensor Technologies
- Heavy Metals in Plants
- Heavy Metal Exposure and Toxicity
- Advanced biosensing and bioanalysis techniques
- Laser-induced spectroscopy and plasma
- Nanopore and Nanochannel Transport Studies
North Carolina State University
2020-2023
China Pharmaceutical University
2017-2022
State Key Laboratory of Natural Medicine
2022
Mass spectrometry imaging (MSI) is an important analytical technique that simultaneously reports the spatial location and abundance of detected ions in biological, chemical, clinical, pharmaceutical studies. As MSI grows popularity, it has become evident data reporting varies among different research groups between techniques. The lack consistency inherently creates additional challenges comparing intra- inter-laboratory data. In this tutorial, we propose a unified system, SMART, based on...
Biospecimens with nearly flat surfaces on a stage are typically required for laser-based mass spectrometry imaging (MSI) techniques. However, sampling stages rarely perfectly level, and accounting this the need to accommodate non-flat samples requires deeper understanding of laser beam depth focus. In ablation-based MSI methods, is focused top sample surface, ensuring that at focal point or remains within general, focus given related quality (M2 ) wavelength (λ). because applied biological...
Rationale Infrared matrix‐assisted laser desorption electrospray ionization (IR‐MALDESI) utilizes a 2970 nm mid‐IR to desorb samples with depth resolutions ( Z ) on the order of micrometers. Conventionally, 5–20 μm thick tissue sections are used characterize different applications IR‐MALDESI source, but an optimal thickness has not been systematically investigated. Methods Mouse liver was sectioned various thicknesses and analyzed using mass spectrometry imaging (MSI). Height profiles...
Two-dimensional mass spectrometry imaging (2D MSI) experiments mainly involve samples with a flat surface and constant thickness, but some are challenging to section due the texture topography. Herein, we present an MSI method that automatically corrects for discernible height differences across surfaces during experiments. A chromatic confocal sensor was incorporated into infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) system measure sample at location of each...
l -cysteine-functionalized gold nanoparticles have significantly improved the enantioseparation performance of capillary electrochromatography with reliable stability and repeatability.
How to improve the enantiomer separation efficiency of drugs is a hot topic. In this paper, polydopamine (PDA) coating doped with graphene oxide (GO) by physical adsorption was used modify capillary column enhance enantioseparation drugs. electrochromatography (CEC) system, novel carboxymethyl-β-cyclodextrin (CM-β-CD) as chiral selector has completed four basic (propranolol, metoprolol, amlodipine and chlorpheniramine). The optimum conditions were obtained optimizing pH buffer, concentration...
Mass spectrometry imaging is a powerful tool to analyze large number of metabolites with their spatial coordinates collected throughout the sample. However, significant differences in ionization efficiency pose big challenge metabolomic mass imaging. To solve and obtain complete data profile, researchers typically perform experiments both positive negative modes, which time-consuming. In this work, we evaluated use dicationic reagent, 1,5-pentanediyl-bis(1-butylpyrrolidinium) difluoride...
This month’s tutorial walks through the parameters that affect laser ablation-based mass spectrometry imaging (MSI) of non-flat surfaces with ambition making MSI-relevant considerations more accessible to broader community. Specifically, equations for depth focus and minimum spot size are related wavelength beam quality in use. explains differences between ultraviolet infrared MSI platforms relation these properties, as well provides modern approaches field surfaces.