A5027150103- Zebrafish Biomedical Research Applications
- Cellular transport and secretion
- Neurological diseases and metabolism
- Hereditary Neurological Disorders
- Cancer, Hypoxia, and Metabolism
- Congenital heart defects research
- Glycosylation and Glycoproteins Research
- Erythrocyte Function and Pathophysiology
- Sphingolipid Metabolism and Signaling
- Adrenal and Paraganglionic Tumors
- Cancer-related gene regulation
- HIV Research and Treatment
- Nuclear Receptors and Signaling
- Birth, Development, and Health
- Adenosine and Purinergic Signaling
- Cell Adhesion Molecules Research
- interferon and immune responses
- Salivary Gland Tumors Diagnosis and Treatment
- Peptidase Inhibition and Analysis
- RNA regulation and disease
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Enzyme function and inhibition
- Tryptophan and brain disorders
- Computational Drug Discovery Methods
University of Brescia
2015-2024
Brescia University
2021-2022
Weatherford College
2021
University of Göttingen
1999-2000
Intracellular protein transport and sorting by vesicles in the secretory endocytic pathways requires formation of a coat on membrane. The heterotetrameric adaptor complex 1 (AP-1) promotes clathrin-coated at trans-Golgi network. AP-1 interacts with various signals cytoplasmic tails cargo molecules, thus indicating function sorting. We generated mutants γ-adaptin subunit mice to investigate its role post-Golgi vesicle processes. γ-Adaptin-deficient embryos develop until day 3.5 post coitus...
Pantothenate Kinase Associated Neurodegeneration (PKAN) is an autosomal recessive disorder with mutations in the pantothenate kinase 2 gene (PANK2), encoding essential enzyme for Coenzyme A (CoA) biosynthesis. The molecular connection between defects this and neurodegenerative phenotype observed PKAN patients still poorly understood. We exploited zebrafish model to study role played by pank2 during embryonic development get new insight into pathogenesis. orthologue of hPANK2 lies on...
Abstract Mutations in Pantothenate kinase 2 and Coenzyme A (CoA) synthase ( COASY ), genes involved CoA biosynthesis, are associated with rare neurodegenerative disorders brain iron accumulation. We showed that zebrafish pank2 gene plays an essential role vasculature development. Now we extended our study to coasy . The has high level of sequence identity the human ortholog is ubiquitously expressed from earliest stages abrogation its expression led strong reduction content, lethality a...
Adrenocortical carcinoma (ACC) is a rare endocrine malignancy with high risk of relapse and metastatic spread. The actin-bundling protein fascin (FSCN1) overexpressed in aggressive ACC represents reliable prognostic indicator. FSCN1 has been shown to synergize VAV2, guanine nucleotide exchange factor for the Rho/Rac GTPase family, enhance invasion properties cancer cells. Based on those results, we investigated effects inactivation by CRISPR/Cas9 or pharmacological blockade invasive cells,...
Cytosolic sialidase Neu2 has been implicated in myoblast differentiation. Here we observed a significant upregulation of expression during differentiation murine C2C12 myoblasts. This was evidenced both as an increase mRNA steady‐state levels and the cytosolic enzymatic activity. To understand biological significance differentiation, cells were stably transfected with rat cDNA. overexpressing clones characterized by marked decrement cell proliferation capacity to undergo spontaneous also...
In pediatric rhabdomyosarcoma (RMS), elevated Akt signaling is associated with increased malignancy. Here, we report that expression of a constitutively active, myristoylated form Akt1 (myrAkt1) in human RMS RD cells led to hyperactivation the mammalian target rapamycin (mTOR)/70-kDa ribosomal protein S6 kinase (p70S6K) pathway, resulting loss both MyoD and myogenic capacity, an increase Ki67 due high cell mitosis. MyrAkt1 migratory invasive traits, as detected by wound healing, zymography,...
As most new medications, Cabotegravir (CAB) was recently approved as an antiretroviral treatment of HIV infection without in-depth safety information on in utero exposure. Although no developmental toxicity rats and rabbits reported, recent studies demonstrated that CAB decreases pluripotency human embryonic stem cells. exposure effects during development were assessed zebrafish embryos by the Fish Embryo Toxicity test after at subtherapeutic concentrations up to 25× Cmax. Larvae behavior...
In this study, we report the effects of caffeine on angiogenesis in zebrafish embryos both during normal development and after exposure to Fibroblast Growth Factor 2 (FGF2). As markers angiogenesis, measured length width intersegmental vessels (ISVs), performed whole-mount situ hybridization with fli1 cadh5 vascular markers, counted number interconnecting (ICVs) sub-intestinal venous plexus (SIVP). addition, performing yolk membrane (ZFYM) assay microinjection fibroblast growth factor (FGF2)...
Abstract Background Nirmatrelvir, in combination with ritonavir, is one of the first orally available antiviral treatment for coronavirus disease 2019 (COVID‐19). Symptomatic pregnant women are at increased risk severe illness and complications that can affect developing baby. No malformations or lower embryo‐fetal survival have been observed when nirmatrelvir were administered to rats rabbits. Safety evaluation drugs used treating COVID‐19 also pregnancy urgent public health, then this...
Adrenocortical cancer (ACC) is a rare malignancy with dismal prognosis. The treatment includes mitotane and EDP chemotherapy (etoposide, doxorubicin, cisplatin). However, new therapeutic approaches for advanced ACC are needed, particularly targeting the metastatic process. Here, we deepen role of progesterone as potential drug ACC, in line its antitumoral effect other cancers.NCI-H295R, MUC-1, TVBF-7 cell lines were used xenografted zebrafish embryos. Migration invasion studied using...
Disturbance of sphingolipid metabolism may represent a novel therapeutic target in metastatic melanoma, the most lethal form skin cancer. β-Galactosylceramidase (GALC) removes β-galactose from galactosylceramide and other sphingolipids. In this study, we show that downregulation galcb, zebrafish ortholog human GALC, affects melanoblast melanocyte differentiation embryos, suggesting possible role for GALC melanoma. On basis, impact expression murine B16-F10 A2058 melanoma cells was...
The aquatic ecotoxicity of three α-amino acid-derived polyamidoamines (PAAs) was studied using zebrafish embryos as a viable vertebrate model organism. PAAs examined were water-soluble amphoteric polyelectrolytes with primarily negative charge, which efficient flame retardants for cotton. fish embryo acute toxicity test performed PAA water solutions 1.5–500 mg L−1 concentrations showed that did not statistically differ from the control. survival rates indeed >90%, even at highest...
Melia azedarach L. is a Meliaceae that has shown important insecticidal activities. However, few researchers have extensively studied the toxicology of aqueous extracts M. (MAE). Therefore, main objective this study was to characterize phyto-eco-toxicological profile MAE. First, botanical and phytochemical characterization MAE performed using histological, metabolomic multi-analytical approach. Second, toxicological effects on pollinating insects (Apis mellifera ligustica) soil collembola...
Zebrafish embryos are emerging as a model for pharmacological and toxicological studies. We used zebrafish to study the general toxicity cardiovascular effects of eight methylxanthines: aminophylline, caffeine, diprophylline, doxofylline, etophylline, 3-isobutyl-1-methylxanthine (IBMX), pentoxifylline theophylline. Microinjections methylxanthines were performed in 1-2 cell stage analyzed at different time points. Embryotoxicity teratogenicity evaluated understand these compounds. Structural...
Abstract Protein transport between the trans‐Golgi network and endosomes is mediated by vesicles formed adaptor‐protein complex AP‐1, consisting of adaptins γ1, β1, μ1, σ1. Mammalia express μ1A ubiquitously isoform μ1B in polarized epithelia. Mouse γ1 or 'knock out's revealed that AP‐1 indispensable for embryonic development. We isolated from Danio rerio . Analysis expression tissue‐specific either one during embryogenesis adult tissues contrast to their mammalia. transcript was detected...