A5063866411- Metabolomics and Mass Spectrometry Studies
- Genetics, Aging, and Longevity in Model Organisms
- Microbial Metabolic Engineering and Bioproduction
- Gut microbiota and health
- Mass Spectrometry Techniques and Applications
- Circadian rhythm and melatonin
- Biosensors and Analytical Detection
- Plant Toxicity and Pharmacological Properties
- Microfluidic and Capillary Electrophoresis Applications
- Enzyme Structure and Function
- Lipid Membrane Structure and Behavior
- Tryptophan and brain disorders
- Data Analysis with R
- Protein Structure and Dynamics
- Bioinformatics and Genomic Networks
- Advanced Proteomics Techniques and Applications
- Drug-Induced Hepatotoxicity and Protection
- Lipid metabolism and biosynthesis
- Botanical Research and Chemistry
- Advanced Chemical Sensor Technologies
- Photosynthetic Processes and Mechanisms
- Analytical Chemistry and Chromatography
Helmholtz Zentrum München
2021-2025
Hochschule Aalen
2020-2022
Federal Union of German Associations of Pharmacists
2019
Liquid chromatography-mass spectrometry (LC-MS)-based untargeted metabolomics experiments have become increasingly popular because of the wide range metabolites that can be analyzed and possibility to measure novel compounds. LC-MS instrumentation analysis conditions differ substantially among laboratories experiments, thus resulting in non-standardized datasets demanding customized annotation workflows. We present an ecosystem R packages, centered around MetaboCoreUtils, MetaboAnnotation...
Hemoglobin (Hb) constitutes an important protein in clinical diagnostics—both humans and animals. Among the high number of sequence variants, some can cause severe diseases. Moreover, chemical modifications such as glycation carbamylation serve biomarkers for conditions diabetes kidney In routine analysis glycated Hb, variants or other Hb proteoforms interference, resulting wrong quantification results. We present a versatile flexible capillary zone electrophoresis-mass spectrometry...
The identification of lipids is a cornerstone lipidomics, and due to the specific characteristics lipids, it requires dedicated analysis workflows. Identifying novel lipid species for which no reference spectra are available tedious often involves lot manual work. Integrating high-resolution mass spectrometry with enhancements from chromatographic ion mobility separation enables in-depth investigation intact lipids. We investigated phosphorylated glycosphingolipids nematode Caenorhabditis...
Protection against pathogens is a major function of the gut microbiota. Although bacterial natural products have emerged as crucial components host-microbiota interactions, their exact role in microbiota-mediated protection largely unexplored. We addressed this knowledge gap with nematode Caenorhabditis elegans and its microbiota isolate Pseudomonas fluorescens MYb115 that known to protect Bacillus thuringiensis (Bt) infection. find MYb115-mediated depends on sphingolipids (SLs) are derived...
Abstract The transition from inorganic catalysis through minerals to organic by enzymes is a necessary step in the emergence of life. Our work elucidating likely reactions at earliest moments Life, prior existence enzymatic catalysis, exploring essential intersections between nickel bioinorganic chemistry and pterin biochemistry. We used prebiotically-inspired acetylene-containing volcanic hydrothermal experimental environment shed light on efficient formation nickel-organo complexes....
Abstract Pyrrolizidine alkaloids and their corresponding pyrrolizidine alkaloid-N-oxides are secondary plant constituents that became the subject of public concern due to hepatotoxic, pneumotoxic, genotoxic, cytotoxic effects. In contrast well-established analytical separation detection methods, only a few studies have investigated extraction alkaloids/pyrrolizidine from material. this study, we applied pressurized liquid with aim evaluating effect various parameters on recovery alkaloids....
Abstract Protection against pathogens is a major function of the gut microbiota. Although bacterial natural products have emerged as crucial components host-microbiota interactions, their exact role in microbiota-mediated protection largely unexplored. We addressed this knowledge gap with nematode Caenorhabditis elegans and its microbiota isolate Pseudomonas fluorescens MYb115 that known to protect Bacillus thuringiensis (Bt) infection. find MYb115-mediated depends on sphingolipids are...
Abstract Summary We present MobilityTransformR, an R/Bioconductor package for the effective mobility scaling of capillary zone electrophoresis-mass spectrometry (CE-MS) data. It uses functionality from different R packages that are frequently used data processing and analysis in MS-based metabolomics workflows, allowing subsequent use reproducible transformed CE-MS existing workflows. Availability implementation MobilityTransformR is implemented (Version >= 4.2) can be downloaded...
Polar metabolites in Caenorhabditis elegans (C. elegans) have predominantly been analyzed using hydrophilic interaction liquid chromatography coupled to mass spectrometry (HILIC-MS). Capillary electrophoresis (CE-MS) represents another complementary analytical platform suitable for polar and charged analytes.We compared CE-MS HILIC-MS the analysis of a set 60 reference standards relevant C. specifically investigated strengths CE separation. Furthermore, we employed as approach study samples,...
Spectral similarity networks, also known as molecular are crucial in non-targeted metabolomics to aid identification of unknowns aiming establish a potential structural relation between different metabolite features. However, too extensive differences compound structures can lead separate clusters, complicating annotation. To address this challenge, we developed an automated Annotation Propagation through multiple EXperimental Networks (APEX) workflow, which integrates spectral networks with...
Abstract Protection against pathogens is a major function of the gut microbiota. Although bacterial natural products have emerged as crucial components host-microbiota interactions, their exact role in microbiota-mediated protection largely unexplored. We addressed this knowledge gap with nematode Caenorhabditis elegans and its microbiota isolate Pseudomonas fluorescens MYb115 that known to protect Bacillus thuringiensis (Bt) infection. find MYb115-mediated depends on sphingolipids are...