A5019816782- Respiratory Support and Mechanisms
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Pulmonary Hypertension Research and Treatments
- Sepsis Diagnosis and Treatment
- Neonatal Respiratory Health Research
- Adipose Tissue and Metabolism
- Cardiovascular Function and Risk Factors
- Cardiac Arrest and Resuscitation
- Heart Failure Treatment and Management
- Trypanosoma species research and implications
- Mesenchymal stem cell research
- Cardiovascular and exercise physiology
- Exercise and Physiological Responses
- Heart Rate Variability and Autonomic Control
- Asthma and respiratory diseases
- Birth, Development, and Health
- Tissue Engineering and Regenerative Medicine
- Congenital Diaphragmatic Hernia Studies
- Coenzyme Q10 studies and effects
- Diet and metabolism studies
- Vagus Nerve Stimulation Research
- Pleural and Pulmonary Diseases
- Intensive Care Unit Cognitive Disorders
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Cardiomyopathy and Myosin Studies
Universidade Federal do Rio de Janeiro
2016-2025
Universidade Federal Fluminense
2015-2025
National Institute of Science and Technology in Regenerative Medicine
2019-2023
Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
2010-2022
University of Quintana Roo
2009
Universidade de São Paulo
2004
Ministério da Saúde
1999-2003
Microsoft (Brazil)
1999
Universidade do Estado do Rio de Janeiro
1999
Ministério da Saúde
1999
We sought to assess whether the effects of mesenchymal stromal cells (MSC) on lung inflammation and remodeling in experimental emphysema would differ according MSC source administration route. Emphysema was induced C57BL/6 mice by intratracheal (IT) porcine pancreatic elastase (0.1 UI) weekly for 1 month. After last instillation, saline or MSCs (1-105), isolated from either mouse bone marrow (BM), adipose tissue (AD) (L), were administered intravenously (IV) IT. week, euthanized. Regardless...
Ischemic stroke causes brain inflammation, which we postulate may result in lung damage. Several studies have focused on stroke-induced immunosuppression and infection; however, the possibility that strokes trigger inflammation has been overlooked. We hypothesized even focal ischemic might induce acute systemic pulmonary thus altering respiratory parameters, tissue integrity, alveolar macrophage behavior.Forty-eight Wistar rats were randomly assigned to (Stroke) or sham surgery (Sham). Lung...
Experimental research has reported beneficial effects of mesenchymal stromal cell (MSC) therapy in pulmonary arterial hypertension (PAH). However, these studies either were based on prophylactic protocols or assessed basic remodeling features without evaluating possible mechanisms. We analyzed the MSC lung vascular and hemodynamics its mechanisms action monocrotaline (MCT)-induced PAH.Twenty-eight Wistar rats randomly divided into two groups. In PAH group, animals received MCT 60 mg/kg...
Infection with Trypanosoma cruzi induces inflammation, which limits parasite proliferation but may result in chagasic heart disease. Suppressor of cytokine signaling 2 (SOCS2) is a regulator immune responses and therefore participate the pathogenesis T. infection. SOCS2 expressed during infection, its expression partially reduced infected 5-lipoxygenase-deficient [knockout (KO)] mice. In KO mice, there was reduction both parasitemia interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α),...
Physical activity modulates inflammation and immune response in both normal pathologic conditions. We investigated whether regular moderate exercise before the induction of experimental sepsis reduces risk lung distal organ injury survival. One hundred twenty-four BALB/c mice were randomly assigned to two groups: sedentary (S) trained (T). Animals T group ran on a motorized treadmill, at intensity, 5% grade, 30 min/day, 3 times week for 8 wk. Cardiac adaptation was evaluated using...
We hypothesized that bone marrow-derived mononuclear cell (BMDMC) therapy protects the lung and consequently heart in experimental elastase-induced emphysema. Twenty-four female C57BL/6 mice were intratracheally instilled with saline (C group) or porcine pancreatic elastase (E once a week during 4 weeks. C E groups randomized into subgroups receiving (SAL) male BMDMCs (2 × 10(6), CELL) intravenously 3h after first instillation. Compared to E-SAL group, E-CELL showed, at 5 weeks: lower mean...
Many experimental models have been proposed to study the pathophysiological features of emphysema, as well search for new therapeutic approaches acute or chronically injured lung parenchyma. We aimed characterize an emphysema model induced by multiple instillations elastase tracking changes in inflammation, remodeling, and cardiac function after each instillation. Forty-eight C57BL/6 mice were randomly assigned across 2 groups. Emphysema (ELA) animals received 1, 2, 3, 4 intratracheal...
Recruitment maneuvers (RMs) seem to be more effective in extrapulmonary acute lung injury (ALI), caused mainly by sepsis, than pulmonary ALI. Nevertheless, the maintenance of adequate volemic status is particularly challenging sepsis. Since interaction between and RMs not well established, we investigated effects on distal organs presence hypovolemia, normovolemia, hypervolemia a model induced ALI was cecal ligation puncture surgery 66 Wistar rats. After 48 h, animals were anesthetized,...
Obese patients are at higher risk of developing acute respiratory distress syndrome (ARDS); however, their survival rates also compared to those similarly ill non-obese patients. We hypothesized that obesity would not only prevent lung inflammation, but reduce remodeling in moderate endotoxin-induced injury (ALI). Obesity was induced by early postnatal overfeeding Wistar rats which the litter size reduced 3 pups/litter (Obese, n=18); Control animals (n=18) were obtained from unculled...
Abstract Introduction The objective of this work was to evaluate the efficacy placenta-derived mesenchymal stem cell (MSC) therapy in a mouse model myocardial infarction (MI). Since MSCs can be obtained from two different regions human term placenta (chorionic plate or villi), cells both these were compared so that best candidate for could selected. Methods For vitro studies, chorionic (cp-MSCs) and villi (cv-MSCs) extensively characterized their genetic stability, clonogenic differentiation...
A single administration of mesenchymal stromal cells (MSCs) has been shown to reduce lung inflammation in experimental elastase-induced emphysema; however, effects were limited terms lung-tissue repair and cardiac function improvement. We hypothesized that two doses MSCs could induce further cardiovascular by mitigating remodeling a model emphysema induced multiple elastase instillations. aimed comparatively investigate the one versus MSCs, administered 1 week apart, murine emphysema....
This study hypothesized that, in experimental mild acute respiratory distress syndrome, lung damage caused by high tidal volume (VT) could be attenuated if VT increased slowly enough to progressively reduce mechanical heterogeneity and allow the epithelial endothelial cells, as well extracellular matrix of adapt. For this purpose, different strategies approaching maximal were tested.Sixty-four Wistar rats received Escherichia coli lipopolysaccharide intratracheally. After 24 h, animals...
Background Persistent fatigue is one of the most common and debilitating symptoms experienced by patients recovering from COVID-19, contributing significantly to burden “long COVID” or post-COVID-19syndrome. However, underlying pathophysiological mechanisms remain inadequately understood. Few studies have examined association between pulmonary cardiac function, systemic biomarkers, morphological changes in lungs diaphragm. Furthermore, potential influence vaccination on persistence has not...
In Brief BACKGROUND Noninvasive ventilation with continuous positive airway pressure (CPAP) has been used in patients chronic heart failure (CHF), although its effect on exercise tolerance these is unknown. The aim of this study was to determine the effects CPAP outpatients CHF. METHODS Following a double-blind, randomized, crossover, and placebo-controlled protocol, 12 CHF (8 male; 4 female; age 54 ± years; body mass index 27.3 1.8 kg/m2, New York Heart Association Class II, III) underwent...
Background Chagas disease, caused by the protozoan Trypanosoma cruzi (T.cruzi), is a complex disease endemic in Central and South America. It has been gathering interest due to increases non-vectorial forms of transmission, especially developed countries. The objective this work was investigate if adipose tissue-derived mesenchymal stromal cells (ASC) can alter course attenuate pathology mouse model chagasic cardiomyopathy. Methodology/Principal Findings ASC were injected intraperitoneally...
Graphical Abstract Septic diaphragm has impaired morphology and increased thickness that seems to be associated, at least in part, with decreased mitochondrial function related reducing Pgc1 α expression, ATP production, number, quality the CLP mice compared control group.
Conflicting data have reported beneficial effects of crystalloids, hyper-oncotic albumin (20%ALB), and iso-oncotic (5%ALB) in critically ill patients. Although may minimize lung injury, recent studies shown that human lead to kidney damage proportional concentration. In this context, we compared the Ringer's lactate (RL), 20%ALB, 5%ALB, all titrated according similar hemodynamic goals, on pulmonary function, histology, molecular biology experimental acute injury (ALI). Male Wistar rats...