A5027804754- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- RNA Interference and Gene Delivery
- Reproductive biology and impacts on aquatic species
- Cancer, Lipids, and Metabolism
- Radiomics and Machine Learning in Medical Imaging
- Cancer Genomics and Diagnostics
- Effects of Environmental Stressors on Livestock
- Reproductive Physiology in Livestock
- Nanoparticle-Based Drug Delivery
- RNA Research and Splicing
- Selenium in Biological Systems
- Endometrial and Cervical Cancer Treatments
- Nuclear Structure and Function
- Glycosylation and Glycoproteins Research
- Prostate Cancer Treatment and Research
- Ubiquitin and proteasome pathways
- Insect and Pesticide Research
- MicroRNA in disease regulation
- Lung Cancer Research Studies
- Protist diversity and phylogeny
- Mesoporous Materials and Catalysis
- Cancer-related molecular mechanisms research
- Microtubule and mitosis dynamics
University of Newcastle Australia
2017-2025
Hunter Medical Research Institute
2022
The University of Melbourne
2020
Endometrial cancer is one of the most frequently diagnosed gynecological cancers worldwide, and its prevalence has increased by more than 50% over last two decades. Despite understanding major signaling pathways driving growth metastasis endometrial cancer, clinical trials targeting these signals have reported poor outcomes. The heterogeneous nature suspected to be key reasons for failure targeted therapies. In this study, we perform a sequential window acquisition all theoretical fragment...
ABSTRACT Herein, we compare the different experimental regimes used to induce testicular heat stress and summarise their impact on sperm production male fertility. Irrespective of protocol used, scrotal causes loss production. This is first seen 1–2 weeks post stress, peaking 4–5 thereafter. The higher temperature, or longer duration heat, more pronounced germ cell becomes, within extreme cases this leads azoospermia. second, often underappreciated hyperthermia poor‐quality spermatozoa....
Male infertility is a complex condition, and for the most part, all men produce defective spermatozoa, but infertile have tendency to more. Despite attempts classify infertility, there no definitive test. One approach would be use protein biomarkers; however as yet, we still do not understand proteins that are differentially expressed within spermatozoa. As such, took nine (fertility status unknown) used Percoll density gradients isolate population of good- poor-quality sperm. For four these...
Abstract Core-shell mesoporous silica nanoparticles (MSN) have recently emerged as a promising drug delivery system that can be used for loading large quantities of different types drugs or creating stimuli responsive system. However, achieving this unique core-shell morphology with smaller size, high specific surface area, and pore volume is highly challenging. In study, we report the synthesis MSN using triple surfactant assisted soft-templating approach. We show morphological textural...
Abstract Using semen data from 1271 ejaculates (79 different bulls, 11 breeds) we have investigated the variability of quality in cattle living sub-tropical conditions. Modelling shows definitive evidence seasonal variation. Semen same bulls had a 90% “pass rate” for cryopreservation purposes winter, dropping to less than 50% summer. Notably, individual could be classified as either “heat-tolerant” (produce good spermatozoa all year regardless temperature) or “heat-sensitive” (only produce...
The introns of the gene encoding long noncoding RNA (lncRNA) GAS5 host up to 10 C/D box small nucleolar RNAs (snoRNAs). However, whether there is a regulatory and functional relationship between these snoRNAs unknown. Here, we show that expression SNORD80, but not other snoRNAs, parallels regulated alongside in response cellular stress. 2′-O-methylation at A496 site, located within segment complementing conserved RNA-binding region on promotes stability consequent upregulation. This...
Objective: To understand the immediate impact that testicular heat stress has on isolated populations of precursor male germ cells including Spermatocytes and Spermatids. Design: Mice were given pre-cursor immediately isolated. RNA sequencing was performed validated using qPCR. Subjects: This work carried out in adult CD1 mice. Results: Using next-generation 134 differentially expressed transcripts found to be upon exposure hyperthermia, 93% which upregulated. In addition, hyperthermia...
Male infertility is widespread and estimated to affect 1 in 20 men. Although some cases the etiology of condition well understood, for at least 50% men, underlying cause yet be classified. infertility, or subfertility, often diagnosed by looking total sperm produced, motility cells overall morphology. counting spermatozoa their associated routine, morphology assessment highly subjective, mainly because procedure being based on microscopic examination. A failure diagnose male-infertility...
Abstract Cultures of Sertoli cells isolated from 20-day-old mice are widely used in research as substitutes for adult cell cultures. This practice is based on the fact that cease to proliferate and become mature vivo by 16 20 days after birth. However, it important verify whether cultured derived do not ex they have same properties cells. Herein we described an isolation/culture method 10-week-old with > 90% purity. Properties these were then compared those (also purity). By counting,...
The free-radical theory of male infertility suggests that reactive oxygen species produced by the spermatozoa themselves are a leading cause sperm dysfunction, including loss motility. However, field is overshadowed on several fronts, primarily because: i) probes used to measure (ROS) imprecise; and ii) many reports suggesting radicals detrimental function add an exogenous source ROS. Herein, more reliable approach superoxide anion production human based MS analysis used. Furthermore,...
What is the nuclear heterogeneity of high-density purified human spermatozoa typically used for IVF purposes.The data show that while density gradient separation has improved overall sperm population, there still a large degree within these cells.Chromomycin A3 (CMA3) an important DNA binding fluorochrome assessment male-factor fertility. It to predict outcomes on entire ejaculates with very high receiver operating characteristic. Here we CMA3 characterise typical populations would be...
In many mammals, the lipid platelet-activating factor (PAF) has important functions in female reproduction and fertility. This study shows that PAF is present reproductive tissues of mares involved processes related to ovulation early pregnancy.
Non-small cell lung cancer (NSCLC) is a life-threatening associated with higher mortality rate. Despite promising results shown by combination therapies, there remains need for efficient drug delivery materials capable of combining various drugs, imaging agents, and targeting agents to enhance treatment efficacy. In this study, we present the synthesis novel core-shell hollow mesoporous silica nanoparticles (@MSN) bimodal porosity large surface area (694 m2/g) facilitate targeted NSCLC...
After ejaculation, mammalian spermatozoa must undergo a process known as capacitation in order to successfully fertilize the oocyte. Several post-translational modifications occur during capacitation, including sialylation, which despite being limited few proteins, seems be essential for proper sperm-oocyte interaction. Regardless of its importance, date, no single study has ever identified nor quantified glycoproteins bearing terminal sialic acid (Sia) are altered capacitation. Here we...
Abstract Testicular heat stress is a well-known and well-described phenomenon that occurs in mammals possess scrotum. Different models to induce testicular hyperthermia, such as surgical cryptorchidism, hot water bath, scrotal insulation or increased environmental temperature have all shown spermatocytes spermatids are unambiguously affected by high temperature, resulting poor sperm production weeks later. The testis appears be very sensitive fluctuations, even small changes temperatures...
Male subfertility or infertility is a common condition often characterized by men producing low number of sperm with poor quality. To gain insight into this condition, we performed quantitative proteomic analysis semen samples obtained from infertile and fertile men. At least 6 proteins showed significant differences in regulation alternatively spliced isoforms. investigate link between aberrant alternative splicing production poor-quality spermatozoa, overexpressed the hnrnpH/F-orthologue...
Abstract Testicular heat stress is a well-known and well-described phenomenon that occurs in mammals possess scrotum. Different models to induce testicular hyperthermia, such as surgical cryptorchidism, hot water bath, scrotal insulation or increased environmental temperature have all shown spermatocytes spermatids are unambiguously affected by high temperature, resulting poor sperm production weeks later. The testis appears be very sensitive fluctuations, even small changes temperatures...
Abstract The family of CDC2-like kinases (CLKs) play a crucial role in regulating alternative splicing (AS), process fundamental to eukaryotic gene expression and adaptation. Of particular interest, these enzymes exhibit unique responsiveness minor temperature shifts, enabling them modulate AS accordingly. Dysregulated CLK is linked wide variety human diseases, establishing as promising therapeutic targets. Despite the importance CLKs, limited research has explored genetic functional...
Capacitation is an essential post-testicular maturation event endowing spermatozoa with fertilizing capacity within the female reproductive tract, significant for fertility, health, and contraception. By using a human-relevant large animal model, domestic boar, this study focuses on furthering our understanding of involvement ubiquitin-proteasome system (UPS) in sperm capacitation. The UPS universal, evolutionarily conserved, cellular proteome-wide degradation recycling machinery, that has...
Abstract A key challenge for years to come is feeding a rapidly growing human population, whilst lowering the overall costs and impact on environment. In addition, as global temperatures increase, natural resources must be utilised basic means overcome climate-related issues. To this end, improvements bull fertility are recognised major mechanism that address both economical environmental balance. Using semen data from 1271 ejaculates (79 different bull, 11 breeds) we have investigated...
Male subfertility or infertility is a common condition often characterized by men producing low number of sperm with poor quality. To gain insight into this condition, we performed quantitative proteomic analysis semen samples obtained from infertile and fertile men. From dataset, 369 proteins showed consistent changes between the groups. Gene ontology revealed that in nucleosome located alternative splicing were ranked highest. This included several exon skipping regulators (hnrnpH/F,...