A5046635986- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Mitochondrial Function and Pathology
- Plant responses to water stress
- Plant Molecular Biology Research
- Redox biology and oxidative stress
- Metabolomics and Mass Spectrometry Studies
- Microbial Metabolic Engineering and Bioproduction
- Light effects on plants
- Plant Water Relations and Carbon Dynamics
- Plant Gene Expression Analysis
- GABA and Rice Research
- Plant and Biological Electrophysiology Studies
- Plant and animal studies
- Growth and nutrition in plants
- Plant biochemistry and biosynthesis
- bioluminescence and chemiluminescence research
- Plant tissue culture and regeneration
- Biochemical Acid Research Studies
- Agricultural and Food Sciences
- Plant responses to elevated CO2
- Morphological variations and asymmetry
- Greenhouse Technology and Climate Control
- Metabolism and Genetic Disorders
Universidade Federal do Ceará
2016-2025
Max Planck Institute of Molecular Plant Physiology
2012-2019
Universidade Federal de Viçosa
2009-2017
Max Planck Society
2015
Significance The present work extends redox-based change in enzyme activity to the TCA cycle of plant mitochondria. Thioredoxin (TRX) was found regulate enzymes mitochondrial (succinate dehydrogenase and fumarase) an associated with it (ATP-citrate lyase) by modulating thiol redox status. A combination experiments based on mutant carbon isotope labeling analyses provides evidence that flux through this pathway is coordinately modulated TRX at level both mitochondria cytosol. results provide...
Abstract During dark‐induced senescence isovaleryl‐CoA dehydrogenase (IVDH) and D‐2‐hydroxyglutarate ( D‐2HGDH ) act as alternate electron donors to the ubiquinol pool via electron‐transfer flavoprotein/electron‐transfer flavoprotein:ubiquinone oxidoreductase (ETF/ETFQO) pathway. However, role of this pathway in response other stresses still remains unclear. Here, we demonstrated that alternative is associated with tolerance drought Arabidopsis . In comparison wild type (WT) lines...
Transcriptomic and proteomic studies have improved our knowledge of guard cell function; however, metabolic changes in cells remain relatively poorly understood. Here we analysed cell-enriched epidermal fragments from tobacco during light-induced stomatal opening. Increases sucrose, glucose fructose were observed opening the presence sucrose medium while no starch observed, suggesting that elevated levels a consequence rather than breakdown. Conversely, reduction was light- plus...
Stomata control the exchange of CO2 and water vapor in land plants. Thus, whereas a constant supply is required to maintain adequate rates photosynthesis, accompanying losses must be tightly regulated prevent dehydration undesired metabolic changes. Accordingly, uptake or release ions metabolites from guard cells necessary achieve normal stomatal function. The AtQUAC1, an R-type anion channel responsible for malate cells, essential efficient closure. Here, we demonstrate that mutant plants...
Summary Isoform 3 of sucrose synthase ( SUS3 ) is highly expressed in guard cells; however, the precise function SUS this cell type remains to be elucidated. Here, we characterized transgenic Nicotiana tabacum plants overexpressing under control stomatal‐specific KST 1 promoter, and investigated changes metabolism during dark light transition. Guard cell‐specific overexpression led increased activity, stomatal aperture, conductance, transpiration rate, net photosynthetic rate growth....
Malate is a central metabolite involved in multiplicity of plant metabolic pathways, being associated with mitochondrial metabolism and playing significant roles stomatal movements. Vacuolar malate transport has been characterized at the molecular level performed by least one carrier protein two channels Arabidopsis (Arabidopsis thaliana) vacuoles. The absence tonoplast Dicarboxylate Transporter (tDT) tdt knockout mutant was previously an impaired accumulation fumarate leaves. Here, we...
Sucrose has long been thought to play an osmolytic role in stomatal opening. However, recent evidence supports the idea that of sucrose this process is primarily energetic. Here we used a combination aperture assays and kinetic [U-13 C]-sucrose isotope labelling experiments confirm degraded during light-induced opening define fate C released from breakdown. We additionally show addition medium did not enhance The experiment showed consistent 13 enrichment fructose glucose, indicating indeed...
Abstract Photosynthesis and water use efficiency, key factors affecting plant growth, are directly controlled by microscopic adjustable pores in the leaf—the stomata. The size of is modulated guard cells, which rely on molecular mechanisms to sense respond environmental changes. It has been shown that physiology mesophyll cells differs substantially. However, implications these differences metabolism at a genome-scale level remain unclear. Here, we used constraint-based modeling predict...
Retrograde signalling pathways that are triggered by changes in cellular redox homeostasis remain poorly understood. Transformed rice plants deficient peroxisomal ascorbate peroxidase APX4 (OsAPX4-RNAi) known to exhibit more effective protection of photosynthesis against oxidative stress than controls when catalase (CAT) is inhibited, but the mechanisms involved have not been characterized. An in-depth physiological and proteomics analysis was therefore performed on OsAPX4-RNAi CAT-inhibited...
Protein phosphorylation is a well-established post-translational mechanism that regulates protein functions and metabolic pathways. It known several plant mitochondrial proteins are phosphorylated in reversible manner. However, the identities of kinases/phosphatases involved this their roles regulation tricarboxylic acid (TCA) cycle remain unclear. In study, we isolated characterized plants lacking two mitochondrially targeted phosphatases (Sal2 PP2c63) along with pyruvate dehydrogenase...
Thioredoxins (TRXs) are important proteins involved in redox regulation of metabolism. In plants, it has been shown that the mitochondrial metabolism is regulated by TRX system. However, functional significance h2, which found at both cytosol and mitochondria, remains unclear. Arabidopsis plants lacking h2 showed delayed seed germination reduced respiration alongside impaired stomatal mesophyll conductance, without impacting photosynthesis under ambient O2 conditions. an increase...
Thioredoxins (TRXs) are ubiquitous proteins engaged in the redox regulation of plant metabolism. Whilst light-dependent TRX-mediated activation Calvin-Benson cycle enzymes is well documented, role extraplastidial TRXs control mitochondrial (photo)respiratory metabolism has been revealed relatively recently. Mitochondrially located TRX o1 identified as a regulator alternative oxidase, of, or associated with, tricarboxylic acid (TCA) cycle, and dihydrolipoamide dehydrogenase (mtLPD) involved...
Plant atmospheric CO2 fixation depends on the aperture of stomatal pores at leaf epidermis. Stomatal or closure is regulated by changes in metabolism two surrounding guard cells, which respond directly to environmental and internal cues such as mesophyll-derived metabolites. Sucrose has been shown play a dual role during movements. The sucrose produced mesophyll cells can be transported vicinity via transpiration stream, inducing periods high photosynthetic rate. By contrast, breakdown...
2-Oxoglutarate dehydrogenase (OGDH) of the tricarboxylic acid (TCA) cycle is often implied to be inactive in cancer, but this was not experimentally tested. We addressed question through specific inhibition OGDH by succinyl phosphonate (SP). SP action on different cancer cells investigated using indicators cellular viability and reactive oxygen species (ROS), metabolic profiling transcriptomics. Relative sensitivity various changed with increasing exposure could differ ATP- NAD(P)H-based...