Plant Growth Promoting Rhizobacteria (PGPR) is considered as the biological agent for improving plant growth. One Group of PGPR that have an important role in growth promoting Actinomycetes. The objective this study was to isolate and screen Actinomycetes isolated from soybean rhizosphere promoter vitro. Fifty-three isolates successfully been using two media, mainly Humic acid Vitamin Agar (HVA) starch casein agar (SCA). Among 53 isolates, 18 (34%) were able produce IAA range 2.08 ppm 16.70...

Synthesis and Antimicrobial studies of novel Benzimidazole derivativesParmender Singh Rathee,Ritu Dhankar, Sunny Bhardwaj,Monika Gupta, Rakesh Kumar

Endophytic bacteria isolated from medicinal plants are recognized as a source of valuable secondary metabolites.The present study aimed to isolate and evaluate the potential antibacterial, antioxidant, cytotoxic properties endophytic associated with Myristica fatua Houtt, along molecular identification chemical composition analysis most isolate.A total 25 isolates were obtained root, leaf, stem bark M. fatua.16 (64%) exhibited antibacterial activity against at least one target...

This study aimed to explore immune responses and analyze of leukocyte cells induced by the recombinant spike protein with epitope sequences FKNHTSPDV obtained from Indonesian SARS-CoV-2 isolate. The is synthesized PT. Genetic Science Indonesia a purity 95% before being injected subcutaneously into Wistar rats on days 1, 14, 28. Blood sampling was done mice had injections at week 0 that undergone 3 or in sixth obtaining an overview cells. Sera were harvested 14 after each injection determine...

Abstract. Wahyudi AT, Priyanto JA, Fijrina HN, Mariastuti HD, Nawangsih AA. 2019. Streptomyces spp. from rhizosphere soil of maize with potential as plant growth promoter. Biodiversitas 20: 2547-2553. Actinomycete is one group rhizobacteria that plays an important role a This study was aimed to evaluate the Actinomycetes isolated in promoting vitro including their ability produce IAA, promote sprout growth, solubilize phosphate, and grow N-free medium. Thirty isolates have been using spread...

In Indonesia, vibriosis is the main disease in shrimp. This caused by Vibrio sp that may decrease productivity of shrimp cultivation. Thus, exploration for new bioactive compounds as biocontrol agent necessary. Marine sponge-associated bacteria one many sources compounds. The aim this study was to screen marine producing anti-Vibrio sp's Total 12 bacterial isolates (15%) 80 isolated from sponges Hyrtios sp, Verongula sp. and Smenospongia had activity different spectra. hemolytic assay showed...

Abstract The global increase in multidrug-resistant (MDR) bacterial infection has rapidly been gaining concern and leading for investigating new strategies to tackle this problem. In study, the antibacterial potential of 25 soil actinomycetes strain evaluated by initial screening against MDR strains including Escherichia coli M19, Pseudomonas aeruginosa Klebsiella pneumoniae Bacillus subtilis Methicillin Resistant Staphylococcus aureus (MRSA). Among them, three isolates encoded APM-7,...

Aeromonas spp. causes the human diseases including diarrhea, gastroenteritis, and bacteremia. can be found in kitchen sponge, one of reservoirs for food-borne bacterial pathogens. Virulence study vivo animal model is important since mimic manifestasions infections. Omphisa fuscidentalis was chosen alternative virulence model, they are same taxonomical order with well-known infections Galleria mellonella. Bacterial isolation selection sponge used Brain Heart Infusion agar Endo Agar,...

Unexplored soil bacteria isolated from Muna Island, Southeast Sulawesi, Indonesia, provide a new source of active molecules serving as prospective antibacterial agents. In the present research, and antibiofilm activities bacterial isolates obtained Island were evaluated. Based on preliminary screening through antagonism assay, 5 out 15 exhibited various spectra activities. At concentration 80 mg/ml, metabolites extracted two selected isolates, M7 P1 inhibited growth five multidrug-resistant...

Indonesia has a high biodiversity, which can be relied upon as potential source of medicinal materials. One the plants in is Kaempferia galanga, demonstrates various pharmacological properties, including antibacterial, anti-inflammatory, antioxidant, anticancer, and antiangiogenic effects. However, directly extracting active compounds from requires considerable amount biomass. To address this challenge, utilizing endophytic bacteria associated with these presents promising alternative....

Sponge associated bacteria with diverse NRPS-PKS genes could be the alternative source of bioactive compounds. This study aimed to analyze activity as well regulatory mechanism regarding anticancer properties derived from these against cancer cell lines. Extraction bacterial metabolites using ethyl acetate solvent resulted yield ranging 0.003 0.016% (w/v). Six extracts showed markedly cytotoxic 8 human hematopoietic lines, IC50 value 27.24 533.73 μg mL-1 in a dose dependent response,...

Abstract. Inggraini M, Nurfajriah S, Priyanto JA, Ilsan NA. 2021. Antimicrobial susceptibility and molecular species identification of clinical carbapenem-resistant bacteria. Biodiversitas 22: 555-562. Antibiotic is the first option treatment for infectious diseases both in human animal. However, excessive usage misuse antibiotics have driven antibacterial resistances worldwide increasing case antibiotic resistance leads to limited options treatment. This study aimed observe antimicrobial A...

Paederia foetida Linn. is a rich source of active compounds, yet its antioxidant and cytotoxic properties remain poorly studied. This current research aimed to investigate the chemical profiles crude extract derived from P. leaves, fractionated substances, their on MCF-7 cells, breast carcinoma cell. Six substances have been separated in thin layer chromatography (TLC) plate. Five fractions showed properties, as indicated by formation yellowish band TLC plate after spraying with DPPH...

Graptophyllum pictum (L.) Griff is a medicinal shrub belonging to the Acanthaceae family and traditionally used treat various diseases.Therefore, this study aims evaluate pharmaceutical properties phytochemical profiles of methanolic extract G. pictum.The results showed that had potent antioxidant activity against 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals with IC50 values 49.00±3.20 µg/mL 70.18±3.27µg/mL, respectively.It...

Abstract. Budiman DZ, Purwaningtyas WE, Priyanto JA, Putra IP, Nawangsih AA, Wahyudi AT. 2023. Glucanase activity produced by rhizospheric Streptomyces tritolerans ARJ 32 and collinus 38 the analysis of their encoding genes. Biodiversitas 24: 5831-5837. The enzyme ?-1,3-glucanase is capable breaking down glucan components in cell walls phytopathogenic fungi. spp. isolated from maize rhizosphere, are believed to produce enzymes with glucanolytic activity, making them promising candidates for...

Objective: This study was aimed to isolate and screen marine sponge-associated bacteria producing anti-Vibrio compounds identify their from the bacterial extract.
 Methods: Sponge-associated were isolated by spread plate method. Their activity against Vibrio parahaemolyticus, V. harveyi, vulnificus determined dual culture test. Three potential isolates identified based on 16S-rRNA gene analysis. All tested for haemolytic characters in blood agar medium. Anti-Vibrio of most also using...

Bacteria have a tendency to form multicellular biofilms, which adhere surfaces and contain extracellular polymeric substances (EPS). This is accompanied by hydrophobic interactions, are vital in the biofilm attachment process. Oral biofilms contribute range of oral health issues, including gingivitis, dental caries, halitosis. Therefore, this research aimed investigate correlation between growth inhibition hydrophobicity reduction Streptococcus mutants, sanguinis, Lactobacillus acidophilus,...

Ocimum basilicum or basil is commonly consumed raw by Indonesian. However, it easily contaminated pathogenic bacteria. This study aimed to detect Gram-negative bacteria in leaves obtained from three traditional markets Bogor. Isolation and quantification of using serial dilution showed that the average number on surface was 9.6 × 107 CFU/g. Basil second market had highest bacteria, namely 11.3 All isolated have same morphology bacilli, then further purified characterized physiologically. The...

Abstract Soil Streptomyces are filamentous Gram-positive bacteria which were the biggest producer of remarkable bioactive compounds with multiple biological roles. This study aimed to assess antioxidant and cytotoxic activities crude extract derived from 3 soil strains, namely APM-7, APM-11, APM-21, was isolated Muna Islands, Southeast Sulawesi as well profiling its using gas chromatography mass spectrometry (GC-MS). The results indicated that ethyl acetate APM-7 strain showed most potential...

Abstract Essential oils are known and have been tested for their antimicrobial properties. Previous studies the activity of clove galangal essential alone. Thus, purpose this study was to determine alone as well combinations. The screening performed by disc diffusion assay against Gram-positive bacteria Gram-negative bacteria. Both showed Escherichia coli at 10% (v/v) concentration. Furthermore, five combinations were made 1:1, 7:3, 9:1, 3:7, 1:9 ratio. Minimum Inhibitory Concentration (MIC)...