A5009080650- Retinal Development and Disorders
- Axon Guidance and Neuronal Signaling
- Neurogenesis and neuroplasticity mechanisms
- Zebrafish Biomedical Research Applications
- Developmental Biology and Gene Regulation
- Microtubule and mitosis dynamics
- Cellular Mechanics and Interactions
- Hippo pathway signaling and YAP/TAZ
- Protein Kinase Regulation and GTPase Signaling
- Cellular transport and secretion
- Molecular Biology Techniques and Applications
- Photoreceptor and optogenetics research
- Trypanosoma species research and implications
- Congenital heart defects research
- Genetic and Kidney Cyst Diseases
- Research on Leishmaniasis Studies
- Ethics and bioethics in healthcare
- Biological Stains and Phytochemicals
- Advanced Fluorescence Microscopy Techniques
- Pluripotent Stem Cells Research
- Cell Adhesion Molecules Research
- Biotin and Related Studies
- thermodynamics and calorimetric analyses
- Phagocytosis and Immune Regulation
- Genetics, Aging, and Longevity in Model Organisms
Universidad de la República de Uruguay
2015-2024
Institut Pasteur de Montevideo
2014-2024
Hospital de Clínicas
2023
University of Cambridge
2005-2006
Ludwig Cancer Research
2003
In the absence of external cues, neurons in vitro polarize by using intrinsic mechanisms. For example, cultured hippocampal extend arbitrarily oriented neurites and then one these, usually nearest centrosome, begins to grow more quickly than others. This neurite becomes axon as it accumulates molecular components apical junctional complex. All other become dendrites. It is unclear, however, whether vivo, which differentiate within a polarized epithelium, break symmetry similar To investigate...
How the site of axon emergence is specified during neural development not understood. Previous studies disagree on relative importance intrinsic and extrinsic mechanisms. The axons retinal ganglion cells (RGCs) emerge basally in vivo, yet because RGCs develop from polarized neuroepithelial within a environment, disentangling influences challenge. We use time-lapse imaging to demonstrate that Laminin acting directly necessary sufficient orient vivo. contact with basal processes newborn...
ABSTRACT Vertebrate photoreceptors detect light through a large cilium-based outer segment, which is filled with photopigment-laden membranous discs. Surrounding the base of segment are microvilli-like calyceal processes (CPs). Although CP disruption has been associated altered morphology and photoreceptor degeneration, role CPs remains elusive. Here, we used zebrafish as model to characterize CPs. We quantified parameters report strong disparity in coverage between subtypes. length stable...
Retinal ganglion cell (RGC) differentiation in vivo is a highly stereotyped process, likely resulting from the interaction of type-specific transcription factors and tissue-derived signaling factors. The primary cilium, as hub cell, may have role during this process but its presence localization RGC generation, contribution to differentiation, not been previously assessed vivo. In work we analyzed distribution cilia using laser scanning confocal microscopy, well their main ultrastructural...
Divisions that generate one neuronal lineage-committed and self-renewing cell maintain the balance of proliferation differentiation for generation diversity. The asymmetric inheritance apical domains components division machinery has been implicated in this process, might involve interactions with fate determinants regulatory feedback loops an as yet unknown nature. Here, we report dynamics Anillin - essential F-actin regulator furrow component its contribution to progenitor divisions...
A series of fifty arylideneketones and thiazolidenehydrazines was evaluated against Leishmania infantum braziliensis. Furthermore, new simplified thiazolidenehydrazine derivatives were Trypanosoma cruzi. The cytotoxicity the active compounds on non-infected fibroblasts or macrophages established in vitro to evaluate selectivity their anti-parasitic effects. Seven ten had good activity three parasites. IC50 values for T. cruzi spp. ranged from 90 nM-25 µM. Eight multi-trypanocidal (the...
Methyl green has long been known as a histological stain with specific affinity for DNA, although its fluorescent properties have remained unexplored until recently. In this article, we illustrate the method preparing methyl aqueous stock solution, that when diluted can be used very convenient nuclear label fixed cells and tissues. Easy procedures to whole zebrafish chick embryos are detailed, examples of images obtained shown. is maximally excited by red light, at 633 nm, emits relatively...
ABSTRACT Myristoylated alanin‐rich C‐kinase substrate (MARCKS) and MARCKS‐like 1, each encoded by a different gene, comprise very small family of actin‐modulating proteins with essential roles in mammalian neural development. We show here that four genes (two marcks two marcksl1 ) are present teleosts including zebrafish, while ancient actinopterigians, sarcopterigian fishes, chondrichtyans only have two. No were found agnaths or invertebrates. All zebrafish expressed during development, we...
MARCKS (Myristoylated Alanine-Rich C Kinase Substrate) is a peripheral membrane protein, especially abundant in the nervous system, and functionally related to actin organization Ca-calmodulin regulation depending on its phosphorylation by PKC. However, susceptible be phosphorylated several different kinases possible interactions between these phosphorylations have not been fully studied intact cells. In differentiating neuroblasts, as well some neurons, there at least one cell-type specific...
Summary Neurulation involves a complex coordination of cellular movements that are in great part based on the modulation actin cytoskeleton. MARCKS, an F‐actin‐binding protein and major substrate for PKC, is necessary gastrulation neurulation morphogenetic mice, frogs, fish. We previously showed this accumulates at apical region closing neural plate chick embryos, here further explore its role process how it regulated by PKC phosphorylation. activation PMA caused extensive tube closure...
Methyl green has long been known as a histological stain with specific affinity for DNA, although its fluorescent properties have remained unexplored until recently. In this article, we illustrate the method preparing methyl aqueous stock solution, that when diluted can be used very convenient nuclear label fixed cells and tissues. Easy procedures to whole zebrafish chick embryos are detailed, examples of images obtained shown. is maximally excited by red light, at 633 nm, emits relatively...
MARCKS is an actin-modulating protein that can be phosphorylated in multiple sites by PKC and proline-directed kinases. We have previously described a form of this specific for differentiating chick neurons, detected with mAb 3C3. Here, we show antibody binds to only when it at Ser 25. These previous data provide hints possible answer the question why ubiquitous seems essential neural development.
Photoreceptor cells of the vertebrate neural retina originate in neuroepithelium, and like other neurons, must undergo cell body translocation polarity transitions to acquire their final functional morphology, which includes features neuronal epithelial cells. We analyzed this process detail zebrafish embryos using vivo confocal microscopy electron microscopy. progenitors were labeled by transgenic expression enhanced green fluorescent protein under regulation photoreceptor-specific promoter...
Myristoylated alanine-rich kinase C substrate (MARCKS), an actin-binding protein, is involved in several signal transduction pathways. It susceptible to be phosphorylated by protein kinases as and some proline-directed kinases. These phosphorylations differently modulate its functions. We previously showed that a phosphorylation at Ser25 (S25p-MARCKS) chickens signature of this ubiquitous neuron differentiation. To gain insight into the possible involvement MARCKS late retinal histogenesis,...