Non-small cell lung cancer is mostly recognized among other types of with a poor prognosis by cause chemotherapeutic resistance and increased metastasis. Luteolin has been found to decrease However, its underlying mechanisms remain unresolved. The objective this study was examine the effect (and mechanism) luteolin on migration invasion human non-small A549 cells.Cell viability investigated 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Wound healing transwell assays...

Chitooligosaccharide (COS), found in both insects and marine sources, has several bioactivities, such as anti-inflammation antioxidant activities. However, the mechanism of shrimp shell COS on retardation inflammatory effects is limited. Therefore, aim this study to examine aforementioned activities LPS-activated RAW264.7 macrophage cells. significantly improved cell viability at level 500 µg/mL could reduce TNF-α, NO IL-6 generations cells (p < 0.05). Furthermore, ROS formation, NF-κB...

This study aimed to investigate the effect of astaxanthin (ASX) extracted and ASX powder from shrimp (Litopenaeus vannamei) shells on Wistar rats with Alzheimer’s disease, induced by amyloid-β (1-42) peptides. In this task, were divided into eight groups: (1) Control, (2) sham operate, (3) negative control (vehicle) + Aβ1-42, (4) extract+Aβ1-42, (5) commercial (6) (7) blank (8) vitamin E Aβ1-42. All treatments orally administrated for 30 days. At 14- 29-days post injection, animals observed...

Apium graveolens L. is a traditional Chinese medicine prescribed as treatment for hypertension, gout, and diabetes. This study aimed to determine the neuroprotective effects of A. extract against Parkinson's disease (PD) model induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in C57BL/6 mice. Male mice treated with MPTP were orally dosed daily 21 days. Behavioral tests, including rotarod apparatus, narrow beam test, drag grid walk swimming resting tremor evaluation, performed....

BACKGROUND/OBJECTIVES: Re-epithelialization has an important role in skin wound healing.Astaxanthin (ASX), a carotenoid found crustaceans including shrimp, crab, and salmon, been widely used for protection.Therefore, we investigated the effects of ASX on proliferation migration human keratinocyte cells explored mechanism associated with that migration.MATERIAL/METHOD: HaCaT were exposed to 0.25-1 μg/mL ASX.Proliferation keratinocytes was analyzed by using MTT assays flow...

Centella asiatica is widely considered the most important medicinal plant for treating and relieving skin diseases. Recently developed standardized extract of ECa 233 has demonstrated positive effects on wound healing incision burn in rats. However, knowledge associated with mechanism was scare. Therefore, this study aimed to investigate effect underlying molecular mechanisms migration a human keratinocyte cell line (HaCaT) using scratch assay. Formation filopodia, key protein as well...

Parkinson’s disease (PD) is characterized by the progressive degeneration of dopaminergic neurons. The cause PD still unclear. Oxidative stress and mitochondrial dysfunction have been linked to development PD. Luteolin, a non-toxic flavonoid, has become interested in an alternative medicine, according its effects on anti-oxidative anti-apoptosis, although underlying mechanism luteolin not fully elucidated. This study aims investigate whether prevents neurotoxicity induction...

The present study was designed to investigate the effect of nicotine and polyaromatic hydrocarbon compounds on cerebral endothelial cells (CECs). Nicotine treatments from 15 min 5h did not cause any changes in expression localization principal junctional proteins. One day treatment with a relatively high concentration induced decrease tight junction protein ZO-1, occludin, adherens protein, cadherin. Treatment 3 x 10(-5)M phenanthrene for 24h caused redistribution occludin Triton X-100...

Osteoporosis is a serious problem affecting health of the elderly. Drugs (bisphosphonates) applied for treatment are often accompanied by adverse side effects. Thus, fish byproduct-derived peptides, particularly hydrolyzed collagen (HC) from defatted sea bass skin, could be safe source anti-osteoporosis agents. This study aimed to examine effects HC on proliferation and differentiation preosteoblast cells. prepared using papain before Alcalase hydrolysis was determined molecular weight (MW)...

Djenkol (Archidendron Pauciflorum) peel is a source of phenolics with an antioxidative activity. Nevertheless, the high extraction efficacy target compounds crucial for isolation such compounds. Pulsed electric field (PEF)-assisted means that increase via electroporation mechanism. This research aimed to study activities using PEF and investigate anti-inflammation extract (DPE) in RAW264.7 macrophage cells. The at different strengths (E) (4.5 6 kV/cm) times (180, 360, 540 ms) was implemented...

Cricket is a potential proteinaceous source used for protein hydrolysate (PH) preparation, having several biological activities. Nevertheless, cricket has high lipid contents, which are susceptible to oxidation during PH preparation. Thus, ethanol was together with vacuum impregnation (VI) enhance defatting efficacy before Also, bioavailability of the digest after gastrointestinal tract (GIT) digestion via Caco-2 monolayer assessed. powder defatted using 1-4 h. Lipid contents were decreased...

Mannitol, which is a cell-impermeable and nontoxic polyalcohol, has been shown to be useful tool for reversible opening of the blood–brain barrier (BBB). Despite successful clinical trials, molecular mechanism mannitol-induced changes in cerebral endothelial cells (CECs) are poorly understood. For our experiments, we used CECs culture, were treated with different, clinically relevant concentrations mannitol. We found that mannitol induced rapid, concentration-dependent, tyrosine...

Hydrolyzed collagen (HC) from sea bass skin prepared using papain and Alcalase had antioxidant potency could enhance MRC-5 cell proliferation lamellipodia formation. HC can be used as a nutraceutical or functional food ingredient.

A current anti-inflammatory agent often targets the prevention of inflammatory disorder development. The standardized Centella asiatica ECa 233 extract has been previously reported for effect. This study aimed to investigate its effect and mechanisms in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages, through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, nitric oxide (NO) reactive oxygen species (ROS) production enzyme-linked immunosorbent assay (ELISA)...

Atherosclerosis, a noncommunicable disease caused by cholesterol plaque, can cause chronic diseases. The antiplatelet medicines used in its treatment complications. Marine collagen peptides be as natural atherosclerosis remedy. present study investigated the preparation and characterization of hydrolyzed (HC) from jellyfish conjugation with black jelly mushroom extract (BJME). Their cytotoxicity ability to prevent cholesterol-induced endothelial cell injury were also examined. HC was...

Metformin has recently emerged as a key player in promotion of neuroblastoma differentiation and neurite outgrowth. However, molecular mechanisms how metformin promotes cellular have not yet been fully elucidated. In this study, we investigated cell differentiation, via an inhibition proliferation, by culturing SH-SY5Y cells with or without metformin. Pretreatment reactive oxygen species (ROS) scavenger, NAC, revealed that ROS plays crucial role induction differentiation. Cell was observed...

Background: Metformin, an antidiabetic drug, has been previously reported to have anti-cancer activities. However, its role in the control of cancer cell migration remains elusive. Methods: To examine possible effect metformin on cervical cells. The related mechanisms were further determined by immunocytochemistry and Western's blotting assay. Results: results showed that treatment substantially inhibited ability Consistently, filopodia lamellipodia formation depleted after exposure...

Cordycepin has been proposed anti-cancer effects, however, it is unclear whether and how cordycepin affects oral squamous carcinoma cell (OSCC) migration invasion. This study aimed to investigate the effect of on invasion OSCC (HSC-4 cells), its underlying mechanism.Cell viability was measured with MTT assay. Migrative invasive abilities were determined by scratch wound healing, agarose spot transwell assays, respectively. Monodasylcadaverine (MDC) staining, immunofluorescence staining LC3...